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FVIIa | Coagulation factor VIIa (50 ĩg)

AS00 001  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Human
FVIIa | Coagulation factor VIIa (50 ĩg) in the group Antibodies Human Cell Biology / Immunology at Agrisera AB (Antibodies for research) (AS00 001)



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Product Information

Immunogen Recombinant human Factor VIIa (NovoSeven ®, Novo Nordisk  A/S Denmark)
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified IgG in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them, to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Prothrombin assay with human plasma (PT), thrombin generation assay, inhibition assay with Factor VIIa with TF and a chromogenic substrate, inDirect ELISA (I-ELISA)
Recommended dilution 1 : 15 000 (I-ELISA), 14 µg/ml (IL)
Expected | apparent MW

52 kDa (this is MW of Factor VIIa)

Reactivity

Confirmed reactivity Human
Predicted reactivity Primates
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information No significant difference in binding to Factor VII and Factor VIIa.

Antibody is purified to total IgG fraction, followed by purification on specific FVIIa column.
This antibody is purified using Protein G, to obtain a total IgG fraction

Related products

Background

Background Factor VIIa (FVIIa) is a key serine protease involved in the initiation of the coagulation cascade. FVIIa requires tissue factor (TF), a membrane bound protein, as an essential cofactor for maximal activity towards its biological substrates Factor X, Factor IX and Factor VII (FVII).

Product citations

Selected references Lopez-Vilchez et al. (2009). Traffic of rFVIIa through Endothelial Cells and Redistribution into Subendothelium: Implications for a Prolonged Hemostatic Effect. Journal of Coagulation Disorders, October 1: (1). (immunolocalization)
immunogen: Recombinant human Factor VIIa (NovoSeven ®, Novo Nordisk  A/S Denmark)
Reconstitution: For reconstitution add 50 µl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified IgG in PBS pH 7.4.
Format: Lyophilized
Quantity: 50 ĩg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them, to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Prothrombin assay with human plasma (PT), thrombin generation assay, inhibition assay with Factor VIIa with TF and a chromogenic substrate, inDirect ELISA (I-ELISA)
recommended dilution: 1 : 15 000 (I-ELISA), 14 µg/ml (IL)
calculated | apparent molecular mass [kDa]:

52 kDa (this is MW of Factor VIIa)

Confirmed reactivity: Human
predicted reactivity: Primates
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information: No significant difference in binding to Factor VII and Factor VIIa.

Antibody is purified to total IgG fraction, followed by purification on specific FVIIa column.
additional information (application): This antibody is purified using Protein G, to obtain a total IgG fraction
background: Factor VIIa (FVIIa) is a key serine protease involved in the initiation of the coagulation cascade. FVIIa requires tissue factor (TF), a membrane bound protein, as an essential cofactor for maximal activity towards its biological substrates Factor X, Factor IX and Factor VII (FVII).
All references: Lopez-Vilchez et al. (2009). Traffic of rFVIIa through Endothelial Cells and Redistribution into Subendothelium: Implications for a Prolonged Hemostatic Effect. Journal of Coagulation Disorders, October 1: (1). (immunolocalization)

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