Recombinant human Factor VIIa (NovoSeven ®, Novo Nordisk A/S Denmark)
Immunogen affinity purified IgG in PBS pH 7.4.
For reconstitution add 50 µl of sterile water
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them, to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prothrombin assay with human plasma (PT), thrombin generation assay, inhibition assay with Factor VIIa with TF and a chromogenic substrate, inDirect ELISA (I-ELISA)
1 : 15 000 (I-ELISA), 14 µg/ml (IL)
Expected | apparent MW
52 kDa (this is MW of Factor VIIa)