GFP | Green Fluorescence Protein, peroxidase conjugated

Product no: AS15 3001

AS15 3001 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in 10 mM Tris pH 8. Contains 0.02 % sodium azide. Soybean persoxidase conjugated.
    Format: Liquid
    Quantity: 100 µg
    Storage: Store at 4°C or in small aliquotes at -20°C; avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunogold (IG), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 5000-1 : 25 000 (ELISA), 1:50 (IG), 1: 100-1:250 (IHC), 1 : 2000-1 : 10 000 (WB)
  • Reactivity
  • Confirmed reactivity: Native GFP, Recombinant GFP (E,coli), all variants of GFP
  • Additional Information
  • Additional information:

    The antibody is detecting native YFP protein, recombinant protein from E.coli and native GFP-fusion proteins and all variants of GFP.

    Additional information (application):

    Minimal cross-reactivity with E.coli proteins

  • Background
  • Background:

    GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa. This antibody is directly conjugated to soyabean peroxidase.

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