GFP | Green Fluorescence Protein, peroxidase conjugated

AS15 3001 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP

Product Information

Immunogen

highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in 10 mM Tris pH 8. Contains 0.02 % sodium azide. Soybean persoxidase conjugated.

Format Liquid

Quantity 100 µg

Storage Store at 4°C or in small aliquotes at -20°C; avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunogold (IG), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 5000-1 : 25 000 (ELISA), 1:50 (IG), 1: 100-1:250 (IHC), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity Native GFP, Recombinant GFP (E,coli), all variants of GFP

Application examples

Additional information

The antibody is detecting native YFP protein, recombinant protein from E.coli and native GFP-fusion proteins and all variants of GFP.

Minimal cross-reactivity with E.coli proteins

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa. This antibody is directly conjugated to soyabean peroxidase.

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background:	GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa. This antibody is directly conjugated to soyabean peroxidase.
Confirmed reactivity:	Native GFP, Recombinant GFP (E,coli), all variants of GFP
additional information:	The antibody is detecting native YFP protein, recombinant protein from E.coli and native GFP-fusion proteins and all variants of GFP.
additional information (application):	Minimal cross-reactivity with E.coli proteins
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Purity:	Immunogen affinity purified serum in 10 mM Tris pH 8. Contains 0.02 % sodium azide. Soybean persoxidase conjugated.
Quantity:	100 µg
recommended dilution:	1 : 5000-1 : 25 000 (ELISA), 1:50 (IG), 1: 100-1:250 (IHC), 1 : 2000-1 : 10 000 (WB)
storage:	Store at 4°C or in small aliquotes at -20°C; avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunogold (IG), Immunohistochemistry (IHC), Western blot (WB)