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GFP | Green Fluorescence Protein, peroxidase conjugated

AS15 3001 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP

GFP | Green Fluorescence Protein, peroxidase conjugated  in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS15 3001)

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Product Information

Immunogen

highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in 10 mM Tris pH 8 with 0.02 % sodium azide.
Quantity 100 µg
Storage Store at 4°C or in small aliquotes at -20°C;  avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 5000-1 : 25 000 (ELISA), 1: 100-1:250 (IHC), 1 : 2000-1 : 10 000 (WB)

Reactivity

Confirmed reactivity Native GFP, Recombinant GFP (E.coli), all variants of GFP

Application examples

Additional information

Additional information

The antibody is detecting native YFP protein, recombinant protein from E.coli and native GFP-fusion proteins and all variants of GFP.

Minimal cross-reactivity with E.coli proteins

Related products

Related products

AS15 2987 | Anti-GFP (affinity purified), rabbit antibodies

AS15 2999 | Anti-GFP (total IgG), rabbit antibodies

AS15 3000 | Anti-GFP (protein A purified) rabbit antibodies

AS15 2998 | Anti-GFP (total IgY), chicken antibodies


AS15 3002 | GFP (purified recombinant protein)

other tag antibodies

Background

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa. This antibody is directly conjugated to soyabean peroxidase.

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