Anti-GFP11 | C-terminal of GFP
AS23 4999 | Clonality: Polyclonal| Host: Rabbit | Reactivity: Universal antibody to C-terminal part of GFP epitope tag
- Product Info
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Immunogen: KLH-conjugated peptide derived from C-terminal of Green fluorescent protein (GFP), UniProt: P42212 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: Depends upon fusion partner - Application Examples
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1 - MW marker
2 - 0.25 µg of GFP
3 - 0.5 µg of GFP
4- 1 µg of GFP
0.25, 0.5, and 1 µg/well of GFP denatured with 4XLDS at 70°C for 5 min. Protein were separated on NuPAGE Bis-Tris SDS gel and blotted 1h to Invitrogen PVDF (pore size of 0.45 µm), using wet transfer. Blot was blocked with 5% milk 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in TBS-T Blocking. The antibody solution was decanted, and the blot was rinsed briefly, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (AS09 607) diluted to 1:2500 in TBS-T Blocking for 30 minutes/RT with agitation. The blot was washed as above and developed for 2 min with Agrisera BCIP/NBT plus.
Courtesy of Agrisera - Background
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Background: GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS20 4443-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: Green Fluorescence Protein (GFP)