GID1c | Gibberellin receptor GID1C
AS14 2800 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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38 kDa (Arabidopsis)
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20 µg of total protein from Arabidopsis thaliana cauline leaves (C.L.), seedling (S.L.) material or imbibed seeds (I.S.) was extracted in 50 mM Phosphate buffer (pH 7.0) with 1X protease inhibitor (Sigma Aldrich) and was separated on a TGX and KD SDS-PAGE gel (BioRad) and blotted 14 min to PVDF using BioRad semi-dry turbo transfer system. Blots were blocked with 2% ECL advance (GE healthcare) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:75,000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds.
Cauline leaves were collected after bolting but before anthesis, around 4 weeks of age (estimate).
Courtesy of Dr. Amber Hauvermale, Department of Crop and Soil Sciences, Washington State University, USA
Alternative names: AtCXE19, Carboxylesterase 19, GID1-like protein 3, Protein GA INSENSITIVE DWARF 1C.
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