GID1c | Gibberellin receptor GID1C

Product no: AS14 2800

AS14 2800 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: recombinant full-length Arabidopsis thaliana GID1c, UniProt: Q940G6 TAIR: At5g27320
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW:

    38 kDa (Arabidopsis)

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Arabidopsis alpina, Ricinus communis
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot detection using anti-GID1c antibodies

    western blot using anti-GID1c antibodies

    20 µg of total protein from Arabidopsis thaliana cauline leaves (C.L.), seedling (S.L.) material or imbibed seeds (I.S.) was extracted in 50 mM Phosphate buffer (pH 7.0) with 1X protease inhibitor (Sigma Aldrich) and was separated on a TGX and KD SDS-PAGE gel (BioRad) and blotted 14 min to PVDF using BioRad semi-dry turbo transfer system. Blots were blocked with 2% ECL advance (GE healthcare) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:75,000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with high sensitivity chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds.

    Cauline leaves were collected after bolting but before anthesis, around 4 weeks of age (estimate).

    Courtesy of Dr. Amber Hauvermale, Department of Crop and Soil Sciences, Washington State University, USA
  • Additional Information
  • Additional information (application): This antibody is also recognizng GST-GID1,
  • Background
  • Background: GID1c (Gibberellin receptor GID1C) is a gibberellin (GA) receptor ortholog of the rice GA receptor OsGID1 which binds to GA and shows affinity to GA4 and interacts with DELLA proteins in vivo in the presence of GA4.

    Alternative names: AtCXE19, Carboxylesterase 19, GID1-like protein 3, Protein GA INSENSITIVE DWARF 1C.
  • Product Citations
  • Selected references: Hauvermale et al. (2015). Loss of Arabidopsis thaliana seed dormancy is associated with increased accumulation of the GID1 GA hormone receptors. Plant Cell Physiol. 2015 Jul 1. pii: pcv084.
  • Protocols

  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
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    Number of reviews: (1)
    Hilla Schayek | 2016-11-07
    0 | 0
    I've used the antibody a few times according to the protocol and only one time it worked for me. 20 and 30 microgram protein gave a weak signal after ECL. but also a constant non-specific bend appeared.


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AS11 1630 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items