GID1c | Gibberellin receptor GID1C

AS14 2800 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

GID1c | Gibberellin receptor GID1C in the group Plant/Algal Antibodies / Hormones / Gibberellins at Agrisera AB (Antibodies for research) (AS14 2800)


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product information
Background GID1c (Gibberellin receptor GID1C) is a gibberellin (GA) receptor ortholog of the rice GA receptor OsGID1 which binds to GA and shows affinity to GA4 and interacts with DELLA proteins in vivo in the presence of GA4. Alternative names: AtCXE19, Carboxylesterase 19, GID1-like protein 3, Protein GA INSENSITIVE DWARF 1C.
Immunogen recombinant full-length Arabidopsis thaliana GID1c, UniProt: Q940G6 TAIR: At5g27320

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

38 kDa (Arabidopsis)

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis alpina, Ricinus communis
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody is also recognizng GST-GID1. 

Selected references Hauvermale et al. (2015). Loss of Arabidopsis thaliana seed dormancy is associated with increased accumulation of the GID1 GA hormone receptors. Plant Cell Physiol. 2015 Jul 1. pii: pcv084.

application example

western blot detection using anti-GID1c antibodies

western blot using anti-GID1c antibodies

20 µg of total protein from Arabidopsis thaliana cauline leaves (C.L.), seedling (S.L.) material or imbibed seeds (I.S.) was extracted in 50 mM Phosphate buffer (pH 7.0) with 1X protease inhibitor (Sigma Aldrich) and was separated on a TGX and KD SDS-PAGE gel (BioRad) and blotted 14 min to PVDF using BioRad semi-dry turbo transfer system. Blots were blocked with 2% ECL advance (GE healthcare) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, Agrisera, AS09 602) diluted to 1:75,000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Advance chemilum (GE healthcare) according to the manufacturer's instructions. Exposure time was 30 seconds.

Cauline leaves were collected after bolting but before anthesis, around 4 weeks of age (estimate).

Courtesy of Dr. Amber Hauvermale, Department of Crop and Soil Sciences, Washington State University, USA

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