RGA | DELLA protein RGA
AS11 1630 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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64 | 64 kDa
40 µg of total protein from 5-d-old dark-grown Arabidopsis thaliana seedlings extracted with 50 mM Tris-HCl pH 7.5,, 10% glycerol, 150 mM NaCl, 0.1% NP-40, 1 mM PMSF, and 1x protease inhibitor cocktail (Roche) were separated on 4-20 % SDS-PAGE and blotted 1h to PVDF. m: mock, P: paclobutyrazol, P+G: PAC+GAs. Blots were blocked with 2% blocking reagent (GE Healthcare) in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602 ) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescence detection reagent, according to the manufacturers instructions. Exposure time was 20 seconds in a LAS-3000 Imager (Fuji).
Courtesy of Dr. David Alabadi, IBMCP (CSIC-UPV), Valencia, Spain
Courtesy of Kamila Jaronczyk, IBB, Warsaw, Poland
RGA protein is prone to degradation therefore caution has to be taken during protein extraction.
Ferrero et al. (2019). Class I TCP transcription factors target the gibberellin biosynthesis gene GA20ox1 and the growth promoting genes HBI1 and PRE6 during thermomorphogenic growth in Arabidopsis. Plant Cell Physiol. 2019 Jul 11. pii: pcz137. doi: 10.1093/pcp/pcz137.
Lorrai et al. (2018). Abscisic acid inhibits hypocotyl elongation acting on gibberellins, DELLA proteins and auxin. AoB Plants. 2018 Oct 5;10(5):ply061. doi: 10.1093/aobpla/ply061.
Chahtane et al. (2018). The plant pathogen Pseudomonas aeruginosa triggers a DELLA-dependent seed germination arrest in Arabidopsis. Elife. 2018 Aug 28;7. pii: e37082. doi: 10.7554/eLife.37082.
Shahnejat-Bushehri et al. (2016). Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. NATURE PLANTS 2: Article number: 16013, 2016.
Crocco et al. (2015). The transcriptional regulator BBX24 impairs DELLA activity to promote shade avoidance in Arabidopsis thaliana. Nat Commun. 2015 Feb 6;6:6202. doi: 10.1038/ncomms7202.
Leone et al. (2014). To grow or defend? Low red : far-red ratios reduce jasmonate sensitivity in Arabidopsis seedlings by promoting DELLA degradation and increasing JAZ10 stability. New Phytol. 2014 Oct;204(2):355-67. doi: 10.1111/nph.12971. Epub 2014 Aug 7.