Glucuronoxylan (monoclonal, clone LM28)

AS18 4208-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom

Product Information

Immunogen Polysaccharide Glucuronoxylan

Host Rat

Clonality Monoclonal

Subclass/isotype IgM

Purity Cell culture supernatant.

Format Liquid

Quantity 1 ml

Storage Store at +4°C (short term) and at -20°C (long term).

Tested applications ELISA (ELISA), Immuniflourescence (IF)

Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Contains 0.05% Sodium Azide.

This antibody is made using a complex pectic immunogen.
It can recognise glucuronosyl substituted xylans and MeGlcA is not required for recognition.

Background

Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Glucuronoxylans are hemicellulosic plant cell wall polysaccharides which contains glucuronic acid and xylose.

Product citations

Selected references Cornault et al. (2015). Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls. Planta. 2015 ec;242(6):1321-34.doi: 10.1007/s00425-015-2375-4.

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Clonality:	Monoclonal
Format:	Liquid
Host:	Rat
immunogen:	Polysaccharide Glucuronoxylan
Purity:	Cell culture supernatant.
Quantity:	1 ml
recommended dilution:	1:10 (ELISA, IF)
storage:	Store at +4°C (short term) and at -20°C (long term).
Sub class:	IgM
tested applications:	ELISA (ELISA), Immuniflourescence (IF)
Confirmed reactivity:	Higher plants, ferns and mosses
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Contains 0.05% Sodium Azide. This antibody is made using a complex pectic immunogen. It can recognise glucuronosyl substituted xylans and MeGlcA is not required for recognition.
background:	The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Glucuronoxylans are hemicellulosic plant cell wall polysaccharides which contains glucuronic acid and xylose.
All references:	Cornault et al. (2015). Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls. Planta. 2015 ec;242(6):1321-34.doi: 10.1007/s00425-015-2375-4.