Anti-Heteroxylan (monoclonal, clone LM11)
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
- Product Info
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Immunogen: Neoglycoprotein (xylopentaose-BSA), Heteroxylan Sub class: IgM Host: Rat Clonality: Monoclonal Purity: Cell culture supernatant. Format: Liquid Quantity: 1 ml Storage: Store at +4°C (short term) and at -20°C (long term). Tested applications: ELISA (ELISA), Immuniflourescence (IF) Recommended dilution: 1:10 (ELISA, IF) - Reactivity
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Confirmed reactivity: Higher plants, ferns and mosses Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Contains 0.05% Sodium Azide.
Binds strongly to wheat arabinoxylan and it can recognise unsubstituted and relatively low-substituted xylans. - Background
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Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Xylan is a group of hemicelluloses in plant cells walls and can also be found in some algae. - Product Citations
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Selected references: McCartney et al. (2005). Monoclonal antibodies to plant cell wall xylans and arabinoxylans. J Histochem Cytochem. 2005 Apr;53(4):543-6.doi: 0.1369/jhc.4B6578.2005. - Protocols
- Immunofluorsecence as described in: Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
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Accessories
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