Goat anti-Human IgG Fc, F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to bovine,mouse,rabbit serum

AS10 1099 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG Fc

Product Information

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified IgG, F(ab)'2 fragment.

Format Liquid

Quantity 0,5 mg

Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.

Recommended dilution 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC),

Reactivity

Application examples

Additional information

This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.

Minimum cross-reactivity is observed with the light chains on human immunoglobulins or to non-immunoglobulin human serum proteins based on immunoelectrophoresis. No reactivity is observed to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. 1% (w/v) BSA, Protease/IgG free.

Background

Background Goat anti-human IgG Fc, F(ab)'2 fragment, FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to human IgG Fc constant part in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,mouse,rabbit serum with a purify of >90% based on SDS-PAGE. Small amounts of intact IgG may be present.

Product citations

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