Goat anti-Mouse IgG2, FITC conjugated

Name: Goat anti-Mouse IgG2, FITC conjugated
Brand: IKP
SKU: AS15 3052
Price: 387 EUR
Availability: InStock

AS15 3052 | Clonality: Polyclonal | Host: Goat | Reactivity:Mouse IgG2

Goat anti-Mouse IgG2, FITC conjugated in the group Secondary Antibodies / Anti-Mouse / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS15 3052)

Product Information

Immunogen Purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b,

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution, add 1 ml of sterile distilled water

Storage Stored lyophilize material at 4°C and for long time storage at -20°C. After reconstirution make aliquots and Store at -20°C to avoid repeated freeze-thaw cycles. Prior to use, thaw the aliquot slowly in the dark at ambient temperature, spin down again and prepare the working dilutions by adding sterile phosphate buffered saline (PBS, pH 7,2). Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day.

Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)(frozen), Immunofluorescence (IF)

Recommended dilution 1 : 20-1 :80 The optimum working dilution of each conjugate should be established by titration before being used, Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal

Reactivity

Confirmed reactivity Mouse IgG2a and IgG2b, subclass specific

Not reactive in It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion

Application examples

Additional information

If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

The reactivity of this product is directed to the subclasses IgG2a and IgG2b as tested against mouse sera other purified homogenous immunoglobulins.

Background

Background Goat anti-mouse IgG2, FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which is subclass specific and binds to mouse IgG1 in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody.

Product citations

immunogen:	Purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b,
Reconstitution:	For reconstitution, add 1 ml of sterile distilled water
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Lyophilized
Quantity:	10 mg
storage:	Stored lyophilize material at 4°C and for long time storage at -20°C. After reconstirution make aliquots and Store at -20°C to avoid repeated freeze-thaw cycles. Prior to use, thaw the aliquot slowly in the dark at ambient temperature, spin down again and prepare the working dilutions by adding sterile phosphate buffered saline (PBS, pH 7,2). Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day.
tested applications:	ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)(frozen), Immunofluorescence (IF)
recommended dilution:	1 : 20-1 :80 The optimum working dilution of each conjugate should be established by titration before being used, Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal
Confirmed reactivity:	Mouse IgG2a and IgG2b, subclass specific
not reactive in:	It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion
additional information:	If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. The reactivity of this product is directed to the subclasses IgG2a and IgG2b as tested against mouse sera other purified homogenous immunoglobulins.
background:	Goat anti-mouse IgG2, FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which is subclass specific and binds to mouse IgG1 in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody.

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