IKP

Goat anti-Mouse IgG2, FITC conjugated

Product no: AS15 3052

AS15 3052   | Clonality: Polyclonal  |  Host: Goat   |  Reactivity:Mouse IgG2

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  • Product Info
  • Immunogen: Purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b,
    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 10 mg
    Reconstitution: For reconstitution, add 1 ml of sterile distilled water
    Storage: Stored lyophilize material at 4C and for long time storage at -20C. After reconstirution make aliquots and Store at -20C to avoid repeated freeze-thaw cycles. Prior to use, thaw the aliquot slowly in the dark at ambient temperature, spin down again and prepare the working dilutions by adding sterile phosphate buffered saline (PBS, pH 7,2). Working dilutions should be stored at 4C, not refrozen, and preferably used the same day.
    Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)(frozen), Immunofluorescence (IF)
    Recommended dilution: 1 : 20-1 :80 The optimum working dilution of each conjugate should be established by titration before being used, Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal
  • Reactivity
  • Confirmed reactivity: Mouse IgG2a and IgG2b, subclass specific
    Not reactive in: It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion
  • Additional Information
  • Additional information:

    If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

    The reactivity of this product is directed to the subclasses IgG2a and IgG2b as tested against mouse sera other purified homogenous immunoglobulins.

  • Background
  • Background: Goat anti-mouse IgG2, FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which is subclass specific and binds to mouse IgG1 in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody.
  • Protocols
  • Antibody protocols
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