Goat anti-Rabbit IgG (H&L) -F(ab)'2 fragment, DyLight® 650 conjugated, min. cross-reactivity to bovine, human, or Mouse lgG or serum proteins

AS12 2337 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L) - F(ab)'2 fragment

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product information
Background		Goat anti-rabbit IgG (H&L) -F(ab)'2 fragment, DyLight®650 Conjugated, min. cross-reactivity to bovine, human, or mouse lgG or serum proteins is a secondary antibody conjugated to DyLight® 650, which binds to Rabbit IgG (H&L) - F(ab)'2 fragment in immunological assays. DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Rabbit IgG DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Immunogen		Purified Rabbit IgG, whole molecule
Host		Goat
Clonality		Polyclonal
Clone
Purity		Affinity purified goat IgG
Format		Lyophilized
Quantity		0.5 mg
Reconstitution		For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products		other dylight anti-Rabbit secondary antibodies
Additional information		Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, human or mouse serum proteins from bovine, human or mouse

application information
Recommended dilution		1 : 20-1 : 2000 for most applications
Expected \| apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references		to be added when available

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