Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, Unconjugated

Product no: AS10 846

AS10 846 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Rabbit IgG (H&L)

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  • Product Info
  • Immunogen:

    Purified Rabbit IgG, whole molecule

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG, F(ab)'2 fragment.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA)
    Recommended dilution: The optimal working dilution should be determined by the investigator
  • Reactivity
  • Confirmed reactivity: Rabbit IgG heavy and light chains (H&L)
    Predicted reactivity: Rabbit IgG Heavy and Light chains (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.

    Purity is ≥ 90 % based on SDS-PAGE. May contain small amounts of intact IgG.

    Additional information (application):

    This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

    No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

  • Background
  • Background:

    Goat anti-rabbit IgG (H&L), F(ab)'2 fragment  is a secondary antibody  which binds to rabbit IgG in immunological assays.

  • Protocols
  • Antibody protocols
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