Goat anti-Rabbit IgG (H&L) HRP conjugated, min.cross-reactivity to bovine/Human/mouse IgG/serum

AS10 668 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Rabbit IgG (H&L)

Goat anti-Rabbit IgG (H&L) HRP conjugated, min.cross-reactivity to bovine/Human/mouse IgG/serum in the group Human/Animal Antibodies / Secondary Antibodies/Blocking / Anti-rabbit  / HRP (horseradish peroxidase) at Agrisera AB (Antibodies for research) (AS10 668)


186 €

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product information

Goat anti-rabbit IgG  is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.


purified Rabbit IgG, whole molecule

Host Goat
Clonality Polyclonal
Purity Purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Related products

AS09 607 | Goat anti-rabbit IgG (H&L) AP conjugated

AS09 608 | Goat anti-rabbit IgG (H&L) biotin conjugated

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.

application information
Recommended dilution 1 : 500 -1 : 5000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 500 -1 : 5000 (WB)
Expected | apparent MW
Confirmed reactivity heavy chains of Rabbit IgG. Light chains on all Rabbit immunoglobulins
Predicted reactivity Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Based on immunoelectrophoresis no reactivity is observed to:

non-immunoglobulin rabbit serum proteins

serum from bovine, human or mouse

IgG from human or mouse

Selected references

to be added when available

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