Goat anti-Rabbit IgG (H&L) HRP conjugated, min,cross-reactivity to bovine/Human/mouse IgG/serum
AS10 668 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
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purified Rabbit IgG, whole molecule
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free
0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
Based on immunoelectrophoresis no reactivity is observed to:
non-immunoglobulin rabbit serum proteins
serum from bovine, human or mouse
IgG from human or mouse
Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.
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