Goat anti-Rabbit IgG (H&L) HRP conjugated, min.cross-reactivity to bovine/Human/mouse IgG/serum

AS10 668 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)


product information
Background		Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.
Immunogen		purified Rabbit IgG, whole molecule
Host		Goat
Clonality		Polyclonal
Clone
Purity		Purified goat IgG
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Related products		AS09 607 \| Goat anti-rabbit IgG (H&L) AP conjugated AS09 608 \| Goat anti-rabbit IgG (H&L) biotin conjugated
Additional information		HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.

application information
Recommended dilution		1 : 500 -1 : 5000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 500 -1 : 5000 (WB)
Expected \| apparent MW
Confirmed reactivity		heavy chains of Rabbit IgG. Light chains on all Rabbit immunoglobulins
Predicted reactivity		Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		Based on immunoelectrophoresis no reactivity is observed to: non-immunoglobulin rabbit serum proteins serum from bovine, human or mouse IgG from human or mouse
Selected references		to be added when available

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