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Goat anti-Rabbit IgG (H&L) HRP conjugated, min.cross-reactivity to bovine/Human/mouse IgG/serum

AS10 668 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Rabbit IgG (H&L)

Goat anti-Rabbit IgG (H&L) HRP conjugated, min.cross-reactivity to bovine/Human/mouse IgG/serum in the group Secondary Antibodies / Anti-Rabbit / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 668)

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Product Information

Immunogen

purified Rabbit IgG, whole molecule

Host Goat
Clonality Polyclonal
Purity Purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500 -1 : 5000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 500 -1 : 5000 (WB)

Reactivity

Confirmed reactivity heavy chains of Rabbit IgG. Light chains on all Rabbit immunoglobulins
Predicted reactivity Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.

Based on immunoelectrophoresis no reactivity is observed to:

non-immunoglobulin rabbit serum proteins

serum from bovine, human or mouse

IgG from human or mouse

Related products

Related products

AS09 607 | Goat anti-rabbit IgG (H&L) AP conjugated

AS09 608 | Goat anti-rabbit IgG (H&L) biotin conjugated

Background

Background

Goat anti-rabbit IgG  is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.

Product citations

Selected references Lacour et al. (2019). Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis. Polar Biol (2019). https://doi.org/10.1007/s00300-019-02507-2.

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