Goat anti-Rabbit IgG (H&L) HRP conjugated, min,cross-reactivity to bovine/Human/mouse IgG/serum

AS10 668 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Product Information

Immunogen

purified Rabbit IgG, whole molecule

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 500 -1 : 5000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 500 -1 : 5000 (WB)

Reactivity

Confirmed reactivity heavy chains of Rabbit IgG, Light chains on all Rabbit immunoglobulins

Predicted reactivity Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.

Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)

Based on immunoelectrophoresis no reactivity is observed to:

non-immunoglobulin rabbit serum proteins

serum from bovine, human or mouse

IgG from human or mouse

Background

Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.

Product citations

Selected references Lacour et al. (2019). Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis. Polar Biol (2019). https://doi.org/10.1007/s00300-019-02507-2.

Confirmed reactivity:	heavy chains of Rabbit IgG, Light chains on all Rabbit immunoglobulins
predicted reactivity:	Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase. Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
additional information (application):	Based on immunoelectrophoresis no reactivity is observed to: non-immunoglobulin rabbit serum proteins serum from bovine, human or mouse IgG from human or mouse
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
immunogen:	purified Rabbit IgG, whole molecule
Purity:	Immunogen affinity purified goat IgG.
Quantity:	1 mg
recommended dilution:	1 : 500 -1 : 5000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 500 -1 : 5000 (WB)
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
All references:	Lacour et al. (2019). Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis. Polar Biol (2019). https://doi.org/10.1007/s00300-019-02507-2.
background:	Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.

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