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Goat anti-Rabbit IgG (H&L), TRITC conjugated, min, cross-reactivity to human serum
- Product Info
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Immunogen: Purified rabbit IgG (H&L) Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution, add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Spin centrifuge shortly to remove any particles. Prepare fresh working dilutions daily. Storage: Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard. Recommended dilution: The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications - Additional Information
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Additional information: Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rabbit serum proteins, human serum proteins.
- Background
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Background: Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm - Protocols
- Antibody protocols
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