Choose currency:
ANTIBODY SHOP
Name:
Phone:
E-mail:
Address:
Send message

GPX | Glutathione peroxidase (chloroplastic)

AS04 055  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

GPX | Glutathione peroxidase (chloroplastic) in the group Antibodies Plant/Algal / Environmental Stress / Plant Oxidative Stress at Agrisera AB (Antibodies for research) (AS04 055)
GPX | Glutathione peroxidase (chloroplastic)



DATA SHEET IN PDF

Qty: 
394 €
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of Arabidopsis thaliana GPX7 accession At4g31870 has been used for antibody production
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 1, 1 : 2, 1 :4, 1 :8, 1 : 16 (ELISA), 1 : 5000 (WB)
Expected | apparent MW

25 | 20 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

Related products

Background

Background

GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.

Product citations

Selected references Lepistö et al. (2013). Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods. J Exp Bot. July 23.
Juszczak et al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant.
immunogen:

thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of Arabidopsis thaliana GPX7 accession At4g31870 has been used for antibody production
Reconstitution: For reconstitution add 100 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 100 ĩl
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: ELISA (ELISA), Western blot (WB)
recommended dilution: 1 : 1, 1 : 2, 1 :4, 1 :8, 1 : 16 (ELISA), 1 : 5000 (WB)
calculated | apparent molecular mass [kDa]:

25 | 20 kDa
Confirmed reactivity: Arabidopsis thaliana
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information (application):

This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.
background:

GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.
All references: Lepistö et al. (2013). Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods. J Exp Bot. July 23.
Juszczak et al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant.

Related products: GPX | Glutathione peroxidase (chloroplastic)

Agrisera ECL kit (Bright/SuperBright)
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
Info Add to cart
AgriseraECL SuperBright
AS16 ECL-S | extreme low femtogram detection

This product can be purchased in 3 different volum...
From 16 €
Info Add to cart
Goat anti-Rabbit IgG (H&L), ALP conjugated
AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until...
206 €
Info Add to cart
Goat anti-Rabbit IgG (H&L), HRP conjugated
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €
Info Add to cart