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GSNOR | S-nitrosoglutathione reductase

AS09 647  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Brassica napus, Helianthus annuus

GSNOR | S-nitrosoglutathione reductase in the group Antibodies for Plant/Algal  / Environmental Stress / Wounding at Agrisera AB (Antibodies for research) (AS09 647)

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Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen

Overexpressed, full length GSNOR derived from Arabidopsis thaliana Q96533, At5g43940

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

40.7 | 40.7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica napus, Helianthus annuus
Predicted reactivity Brassica napus, Oryza sativa, Pisum sativum, Populus balsamifera, Ricinus communis, Solanum tuberosum, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples
Application example

Western blot using anti-GNSOR antibody
2 µg of total protein from (1) Arabidopsis thaliana WT and GSNOR null mutant were separated on 7.5%SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubatedanti-GSNOR antibodies at a dilution of 1: 1 000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).

Additional information

Additional information

Antibody is easily detecting GSNOR in a load per well of 5 µg of total Arabidopsis thaliana cell extract

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Background

Background

S-nitrosoglutathione reductase (GSNOR) is a cytoplasm localized enzyme which plays a key role in formaldehyde detoxification and is down regulated by wounding and activated by salicylic acid (SA).
Alternative protein names: Alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, FALDH, FDH formaldehyde dehydrogenase, alcohol dehydrogenase III, HOT5, S-(hydroxymethyl)glutathione dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH

Product citations

Selected references Feigl et al. (2019). Zinc-induced root architectural changes of rhizotron-grown B. napus correlate with a differential nitro-oxidative response. Nitric Oxide. 2019 Sep 1;90:55-65. doi: 10.1016/j.niox.2019.06.003.
Jain et al. (2018). S-nitrosylation/denitrosylation as a regulatory mechanism of salt stress sensing in sunflower seedlings. Physiol Plant. 2018 Jan;162(1):49-72. doi: 10.1111/ppl.12641.
Kovacs et al. (2016). ROS-Mediated Inhibition of S-nitrosoglutathione Reductase Contributes to the Activation of Anti-oxidative Mechanisms.Front. Plant Sci., 10 November 2016
McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
Zhou et al. (2016). Arabidopsis CaM1 and CaM4 Promote Nitric Oxide Production and Salt Resistance by Inhibiting S-Nitrosoglutathione Reductase via Direct Binding. PLoS Genet. 2016 Sep 29;12(9):e1006255. doi: 10.1371/journal.pgen.1006255. eCollection 2016.
Lee et al. (2008). Modulation of nitrosative stress by S-nitrosoglutathione reductase is critical for thermotolerance and plant growth in Arabidopsis. The Plant Cell 20: 786-802.

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