GST1 | Glutathione-S-transferase (algal)
AS15 2883 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
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Product Information
Immunogen
KLH-conjugated synthetic peptide derived from Glutathione-S-transferase protein sequence from Chlamydomonas reinhardtii, UniProt: A8JBA7
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000 (WB)
Expected | apparent MW
23,9 kDa
Reactivity
Confirmed reactivity
Chlamydomonas reinhardtii
Predicted reactivity
Volvox carteri
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Application examples
Application examples
application example
10 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS/50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed for 30 s with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was typically 30 seconds.
Courtesy Dr. Thomas Roach, University of Innsbruck, Austria
10 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS/50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed for 30 s with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was typically 30 seconds.
Courtesy Dr. Thomas Roach, University of Innsbruck, Austria
Additional information
Background
Background
Glutathione-S-transferase (GST1) is an enzyme which belongs to GST superfamily and has a transferase activity. It is coded by a gene GSTS1.
Product citations
Selected references
Roach et al. (2018). Distress and eustress of reactive electrophiles and relevance to light stress acclimation via stimulation of thiol/disulphide-based redox defences. Free Radic Biol Med. 2018 Mar 18. pii: S0891-5849(18)30134-5. doi: 10.1016/j.freeradbiomed.2018.03.030.
Kumar and Chattopadhyay (2018). Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis. J Exp Bot. 2018 Jun 27;69(15):3729-3743. doi: 10.1093/jxb/ery166.
Kumar and Chattopadhyay (2018). Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis. J Exp Bot. 2018 Jun 27;69(15):3729-3743. doi: 10.1093/jxb/ery166.
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