GST1 | Glutathione-S-transferase (algal)

Product no: AS15 2883

AS15 2883 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Glutathione-S-transferase protein sequence from Chlamydomonas reinhardtii, UniProt: A8JBA7

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW: 23,9 kDa
  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii
    Predicted reactivity: Volvox carteri
    Species of your interest not listed? Contact us
  • Application Examples
  • application example

    western blot using anti-algal GST1 antibodies

    10 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS/50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed for 30 s with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was typically 30 seconds.

    Courtesy Dr. Thomas Roach, University of Innsbruck, Austria
  • Background
  • Background: Glutathione-S-transferase (GST1) is an enzyme which belongs to GST superfamily and has a transferase activity. It is coded by a gene GSTS1.
  • Product Citations
  • Selected references: Roach et al. (2018). Distress and eustress of reactive electrophiles and relevance to light stress acclimation via stimulation of thiol/disulphide-based redox defences. Free Radic Biol Med. 2018 Mar 18. pii: S0891-5849(18)30134-5. doi: 10.1016/j.freeradbiomed.2018.03.030.
    Kumar and Chattopadhyay (2018). Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis. J Exp Bot. 2018 Jun 27;69(15):3729-3743. doi: 10.1093/jxb/ery166.
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
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