Name:
Phone:
E-mail:
Address:






GST1 | Glutathione-S-transferase (algal)

AS15 2883 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

GST1 | Glutathione-S-transferase (algal) in the group Antibodies Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS15 2883)
GST1 | Glutathione-S-transferase (algal)



DATA SHEET IN PDF

Qty: 
302
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Glutathione-S-transferase protein sequence from Chlamydomonas reinhardtii, UniProt: A8JBA7

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW 23,9 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Volvox carteri
Species of your interest not listed? Contact us

Application examples

Application examples application example

western blot using anti-algal GST1 antibodies

10 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS/50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed for 30 s with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was typically 30 seconds.

Courtesy Dr. Thomas Roach, University of Innsbruck, Austria

Additional information

Related products

Background

Background Glutathione-S-transferase (GST1) is an enzyme which belongs to GST superfamily and has a transferase activity. It is coded by a gene GSTS1.

Product citations

Selected references Roach et al. (2018). Distress and eustress of reactive electrophiles and relevance to light stress acclimation via stimulation of thiol/disulphide-based redox defences. Free Radic Biol Med. 2018 Mar 18. pii: S0891-5849(18)30134-5. doi: 10.1016/j.freeradbiomed.2018.03.030.
Kumar and Chattopadhyay (2018). Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis. J Exp Bot. 2018 Jun 27;69(15):3729-3743. doi: 10.1093/jxb/ery166.
immunogen:

KLH-conjugated synthetic peptide derived from Glutathione-S-transferase protein sequence from Chlamydomonas reinhardtii, UniProt: A8JBA7

Reconstitution: For reconstitution add 50 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Quantity: 50 ĩg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 5000 (WB)
calculated | apparent molecular mass [kDa]: 23,9 kDa
Confirmed reactivity: Chlamydomonas reinhardtii
predicted reactivity: Volvox carteri
Species of your interest not listed? Contact us
Picture (footer): application example

western blot using anti-algal GST1 antibodies

10 µg of total protein from Chlamydomonas reinhardtii extracted with 2 % SDS/50 mM TRIS pH 6.8 + protease inhibitor cocktail were separated on 12 % SDS-PAGE and blotted for 1 h to PVDF using semi-dry transfer. Blots were blocked with 5 % low-fat milk powder TBS + 0.1 % Tween for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1 h at RT with agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times each for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in 2 % low-fat milk powder TBS + 0.1 % Tween for 1h at RT with agitation. The blot was washed as above and developed for 30 s with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was typically 30 seconds.

Courtesy Dr. Thomas Roach, University of Innsbruck, Austria
background: Glutathione-S-transferase (GST1) is an enzyme which belongs to GST superfamily and has a transferase activity. It is coded by a gene GSTS1.
All references: Roach et al. (2018). Distress and eustress of reactive electrophiles and relevance to light stress acclimation via stimulation of thiol/disulphide-based redox defences. Free Radic Biol Med. 2018 Mar 18. pii: S0891-5849(18)30134-5. doi: 10.1016/j.freeradbiomed.2018.03.030.
Kumar and Chattopadhyay (2018). Glutathione modulates the expression of heat shock proteins via the transcription factors BZIP10 and MYB21 in Arabidopsis. J Exp Bot. 2018 Jun 27;69(15):3729-3743. doi: 10.1093/jxb/ery166.

Related products: GST1 | Glutathione-S-transferase (algal)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 26 €
AS15 2991 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Sce...
302 €
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €
AS15 2882 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
302 €