H3 | Histone H3, DyLight® 594 conjugated (40 µg)

Product no: AS10 710-DL594
AS10 710-DL594 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardtii, H. vulgare, S. lycopersicum, V. faba, P. patens, S. europaea, Z. mays | cellular [compartment marker] of nucleoplasm
490
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Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030, At4g40040, At5g10980), H3.2 P59226 (At1g09200, At3g27360, At5g10390, At5g10400, At5g65360), H3-like 2 Q9FXI7 (At1g19890)
    Host: Rabbit
    Clonality: Polyclonal
    Purity:

    Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 594.

    Format: Liquid in PBS pH 7,4.
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
    Tested applications: Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: To be determined by end user.
    Expected | apparent MW: 15 | 17 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, Cucurbita pepo L. var. cylindrica, Hordeum vulgare, human, Nicotiana benthamiana, Phalaenopsis equestris, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays
    Predicted reactivity:

    Brachypodium distachyon, Brassica napus, Emiliania huxleyi, Glycine max, Nannochloropsis gaditana, Nicotiana tabacum, Malus domestica, Medicago sativa, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Ostreococcus sp. , Sorghum bicolor, Vitis vinifera, Volvox sp.


    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known,
  • Additional Information
  • Additional information: Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii.

    DyLight® 594 has Amax = 593 nm, Emax = 618 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.

  • Background
  • Background: Histone 3 (H3) located in nuclei, incorporated into chromatin, Present in nucleosome together with H2A, H2B and H4.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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