SUN1,2 (nuclear envelope protein) (Arabidopsis thaliana)

AS15 2856 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SUN1,2 (nuclear envelope protein) (Arabidopsis thaliana) in the group Plant/Algal Antibodies / DNA/RNA/Cell Cycle / Nuclear signaling at Agrisera AB (Antibodies for research) (AS15 2856)


272 €
Buy 2 items of this product for 202 €/items
Buy 3 items of this product for 185 €/items

Info: More information Add review
product information
Background SUN1,2 (nuclear envelope protein) is a member of the Sad1/UNC-84 (SUN)-domain proteins.SUN domain proteins are part of the cytoskeletal-nucleoskeletal bridging complexes. These proteins are localized to the nuclear envelope and are present as homomers and heteromers in vivo. Involved in maintaining the elongated nuclear shape of epidermal cells.

KLH-conjugated synthetic peptide derived from SUN1,2 sequences of Arabidopsis thaliana SUN1 UniProt: Q9FF75, TAIR: AT5G04990, SUN2, UniProt: Q9SG79, TAIR: AT3G10730

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS11 1804 | RNA polymerase II subunit B1, rabbit antibody

AS10 710PRE | H3 | histone H3, pre-immune serum

collection of antibodies to DNA/RNA/cell cycle

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

51.5 kDa (SUN1); 49.9 kDa (SUN2)

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis thaliana ,for other species use product AS15 2857
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Protocol for isolation of cytosolic and nuclear fractions can be found here.

This antibody is not suitable for immunolocalization.

Selected references To be added when available, antibody released in June 2016.

Application example

western blot using anti-SUN1,2 antibodies

Chloroplast fraction was obtained using Chloroplast Isolation Kit (CPISO-1KT, Sigma-Aldrich). 1. Supernatant proteins after DNA digestion, 2. Unquantified, 10 µl of pellet after DNA digestion. Signal of SMT1 (Sterol methyltransferase 1, marker antibody of integral ER membrane) was not detected. Nuclei fraction was isolated from 1 g fresh weight of 3-4 old day cell suspension culture of Arabidopsis thaliana Ler according to protocol published by Xu and Copeland (2012). Nuclei fraction was stored in NSB buffer (with added ROCHE and PMSF inhibitors) in 80 °C. Residue of nuclei was centrifuged and pellet was re-suspended in 100 µl of modified RIPA buffer with added protease inhibitors and incubated 1h on ice. Then 20 µl of DNase I was added into each sample and all samples were incubated on ice for 45 min. After that 45 min another 20 µl of DNase I was added and incubated under the same conditions. Samples were twice centrifuged 1) 15 000 × g for 15 min and 2) 30 000 × g for 30 min. Pellets from the first step of centrifugation was stored. Proteins in second supernatant were precipitated with ice-cold acetone over night and twice washed. Protein pellet was dissolved in buffer contained 6 M Urea, 2 M Thiourea and 25 mM Tris, pH = 7,5. Proteins were quantified by using Bradford method. Totally 3 µg of protein was loaded on gel. Pellet after DNase treatment was dissolved in 20 µl of LSB buffer. Samples were boiled on 95°C for 10 min before loading on 4 / 12% polyacrylamide gel. Electrophoretic conditions: 90 V during whole separation (2 h); Transfer conditions: 90 mA, 30 V over night, nitrocellulose membrane; Blocking: 1 h, 5% low fat milk; Primary antibody incubation: 1 h at a dilution of 1: 1000; Washes: 3 × 5 min.; Secondary antibody: 1 h at a dilution of 1: 5000; washes: 3 × 10 min. ELC substrate was loaded on membrane and signals were detected with ChemiDoc system (Bio-Rad).

Courtesy of Dr. Martin Kubeš, Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Chemical Biology and Genetics, Olomouc, Czech Republic and Vladimír Skalický, Laboratory of Growth Regulators, Faculty of Science of Palacký University and Institute of Experimental Botany ASCR, Olomouc, Czech republic

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at