H3K18me2 | Histone H3 (dimethylated Lys18)

PRODUCT INFORMATION IN PDF

product information Background The di-methylated K18 on histone H3 is a seemingly transient post-translational modification. H3K18 is better known to be acetylated, and occasionally mono-methylated. Suv39h1, a well-studied histone methyltransferase seems to be responsible for the transition of acetylation and methylation at this H3 modification site. The di-methylated K18 on H3 seems to be associated with embryological development and possibly implantation. Immunogen KLH-conjugated synthetic peptide Host Rabbit Clonality Polyclonal Clone Purity Affinity purified serum Format Liquid Quantity 50 µg Reconstitution Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications Chromatin immunoprecipiation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB) Related products AS16 3181 | anti-H3K18me1 | Histone H3 (monomethyl Lys18), rabbit antibodies AS16 3168 | anti-H3T3pK4me1 | Histone H3 (monomethyl Lys4, p Thr3), rabbit antibodies AS16 3169 | anti-H3T3pK4me2 | Histone H3 (dimethyl Lys4, p Thr3), rabbit antibodies AS16 3172 | anti-H3K4me3T6p | H3 (trimethyl Lys4, p Thr6), rabbit antibodies AS16 3197 | anti-anti-H3core, monoclonal antibodies AS15 2855 | anti-H3 | histone H3, chicken polyclonal antibodies AS10 710 | anti-H3 | histone H3, rabbit polyclonal antibodies AS10 710PRE | H3 | histone H3, pre-immune serum to H3 antibody AS10 710 collection of antibodies to epigenetics Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.

application information
Recommended dilution		2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 500 (IF), 1 : 100 (IHC), 1 : 500 (WB)
Expected | apparent MW		15 kDa
Confirmed reactivity		Human
Predicted reactivity		Caenorhabditis elegans, Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		To be added when available, antibody released in February 2016.

application example


Chromatin Immunoprecipitation using anti-H3K18me2 antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3K18me2 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using real-time PCR and normalized to the input chromatin.


Dot Blot using anti-H3K18me2 antibodies. Lane 1: K18 unmodified. Lane 2: K18Me2. Lane 3: K18Me3. Lane 4: K18ac. Lane 5: K18Me1. Load: 0.1, 0.3, and 1 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.


Immunofluorescence using anti-H3K18me2 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:500 dilution for 1 h at RT. Secondary antibody: Dylight® 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K18me2 is nuclear and chromosomal. Staining: Histone H3K18me2 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight® 594 (red).


Western Blot using anti-H3K18me2 antibodies. 30 μg of NIH-3T3 histone extract. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

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