H3R17me2(asym) | Histone H3 (asym-dimethyl Arg17)

AS16 3180 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.

Product Information

Immunogen KLH-conjugated synthetic peptide

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum

Format Liquid

Quantity 50 µg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Western blot (WB)

Recommended dilution 2-5 µg/million cells (ChIP), 1 : 50 (IF), 1 : 500 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Caenorhabditis elegans, Human

Predicted reactivity Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

IF using anti-C.elegns, chicken, D. melanogaster, human, mouse, plant, rat, Xenopus sp. polyclonal antibodies

IF using anti-C.elegns, chicken, D. melanogaster, human, mouse, plant, rat, Xenopus sp. polyclonal antibodies

Chromatin Immunoprecipitation using anti-H3R17me2a antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of H3R17me2a antibody alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.

immunofluorescence using anti-H3R17me2(asym) | Histone H3 (asym-dimethyl Arg17) polyclonal antibodies

Immunofluorescence using anti-H3R17me2a antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3R17me2a is nuclear and chromosomal. Staining: Histone H3R17me2a is expressed in green, nuclei are counterstained with Dapi (blue).

western blot using anti-H3R17me2(asym) | Histone H3 (asym-dimethyl Arg17) polyclonal antibodies

western blot using anti-H3R17me2(asym) | Histone H3 (asym-dimethyl Arg17) polyclonal antibodies

Western Blot anti-H3R17me2a antibodies. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

western blot using anti-H3R17me2(asym) | Histone H3 (asym-dimethyl Arg17) antibodies

western blot using anti-H3R17me2(asym) | Histone H3 (asym-dimethyl Arg17) antibodies

Western Blot using-H3R17me2a antibodies. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.

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Background

Background Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimetylation of H3 Arg17 (H3 R17Me2) has been linked to gene activation. Coactivator-associated arginine methyltransferase-1 (CARM1) methylates Arg17 with its protein arginine methyltransferase (PRMT) catalytic core. Activation of this modification is linked to transcription hormone response promotors, as well as cell fate regulation. Interestingly, H3 methylation of R17 and R26 contributes to greater pluripotency potential of stem cells, while downregulation of this PTM increases differentiation.