H3R17me2(asym) | Histone H3 (asym-dimethyl Arg17)
AS16 3180 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
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Product Information
Immunogen
KLH-conjugated synthetic peptide
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum
Format
Liquid
Quantity
50 µg
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
2-5 µg/million cells (ChIP), 1 : 50 (IF), 1 : 500 (WB)
Expected | apparent MW
15 kDa
Reactivity
Confirmed reactivity
Caenorhabditis elegans, Human
Predicted reactivity
Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
application example
Chromatin Immunoprecipitation using anti-H3R17me2a antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of H3R17me2a antibody alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.
Immunofluorescence using anti-H3R17me2a antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3R17me2a is nuclear and chromosomal. Staining: Histone H3R17me2a is expressed in green, nuclei are counterstained with Dapi (blue).
Western Blot anti-H3R17me2a antibodies. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
Western Blot using-H3R17me2a antibodies. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
Chromatin Immunoprecipitation using anti-H3R17me2a antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of H3R17me2a antibody alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.
Immunofluorescence using anti-H3R17me2a antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3R17me2a is nuclear and chromosomal. Staining: Histone H3R17me2a is expressed in green, nuclei are counterstained with Dapi (blue).
Western Blot anti-H3R17me2a antibodies. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
Western Blot using-H3R17me2a antibodies. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
Additional information
Additional information
This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
Background
Background
Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimetylation of H3 Arg17 (H3 R17Me2) has been linked to gene activation. Coactivator-associated arginine methyltransferase-1 (CARM1) methylates Arg17 with its protein arginine methyltransferase (PRMT) catalytic core. Activation of this modification is linked to transcription hormone response promotors, as well as cell fate regulation. Interestingly, H3 methylation of R17 and R26 contributes to greater pluripotency potential of stem cells, while downregulation of this PTM increases differentiation.
Product citations
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