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H3R17me2(asym) | Histone H3 (asym-dimethyl Arg17)
Product no: AS16 3180
AS16 3180 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
495
€
Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated synthetic peptide Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum. Format: Liquid Quantity: 50 µg Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Chromatin immunoprecipitation (ChIP), Immunofluorescence (IF), Western blot (WB) Recommended dilution: 2-5 µg/million cells (ChIP), 1 : 50 (IF), 1 : 500 (WB) Expected | apparent MW: 15 kDa
- Reactivity
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Confirmed reactivity: Caenorhabditis elegans, Human Predicted reactivity: Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp. Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example 
Chromatin Immunoprecipitation using anti-H3R17me2a antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 ug of H3R17me2a antibody alongside a no antibody (No Ab) control. DNA was measured by qPCR and normalized to total input.
Immunofluorescence using anti-H3R17me2a antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3R17me2a is nuclear and chromosomal. Staining: Histone H3R17me2a is expressed in green, nuclei are counterstained with Dapi (blue).
Western Blot anti-H3R17me2a antibodies. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
Western Blot using-H3R17me2a antibodies. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT. - Additional Information
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Additional information: This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol - Background
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Background: Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimetylation of H3 Arg17 (H3 R17Me2) has been linked to gene activation. Coactivator-associated arginine methyltransferase-1 (CARM1) methylates Arg17 with its protein arginine methyltransferase (PRMT) catalytic core. Activation of this modification is linked to transcription hormone response promotors, as well as cell fate regulation. Interestingly, H3 methylation of R17 and R26 contributes to greater pluripotency potential of stem cells, while downregulation of this PTM increases differentiation. - Reviews:
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