H3K56me3 | Histone H3 (trimethylated Lys56)
AS16 3188 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
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Immunogen KLH-conjugated synthetic peptide
Purity Affinity purified serum
Quantity 50 ĩg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipiation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)
Recommended dilution 2-5 ĩg/million cells (ChIP), 1 :40 (Dot), 1 : 100 (IF), 1 : 500-1 : 1000 (WB)
Expected | apparent MW
Confirmed reactivity Human
Predicted reactivity Caenorhabditis elegans, Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0.01% sodium azide and 30% glycerol.
Background Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Trimethylation of lysine 56 on H3 is a novel modification, but has been reported in mouse cells at very low frequencies.
Selected references To be added when available, antibody released in February 2016.