Thioredoxin-like protein HCF164, chloroplastic
AS16 4097 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Lyophilized antibody can be stored at -20°C for up to 3 years.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
28.6 | 26 kDa
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Arabidopsis thaliana Col0 thylakoids were extracted in isolation buffer (330 mm Sucrose, 25 mm HEPES-KOH, pH 7.4, 10 mm MgCl2, and 10 mm NaF) and detarud with Laemmli buffer at 65C for 10 min. Proteins were separed on 12 % SDS-PAGE and blotted 1 h on a PVDF membrane using semi-dry transfer. Blot was blocked with 8 % milk for 1 h at RT. Blot was incubated in the primary antibody at a dilution of 1: 3 000 overnight at 4°C with agitation in TBS-T with 1% milk. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:25 000 in for 2h at RT with agitation. The blot was washed as above, incubated in chemiluminescent detection reagent for 5 min and developed on film with 1 min exposure.
Courtesy of Msc Lauri Nikkanen, University of Turku, Finland
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