Thioredoxin-like protein HCF164, chloroplastic

272 €
Buy 2 items of this product for 202 €/items
Buy 3 items of this product for 185 €/items

AS16 4097   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana


9 st
Item No:
AS16 4097

Info: Product suggestions Add review
product information
Background Thioredoxin-like protein HCF164, chloroplastic is involved in maintaining cell redox homeostasis. The portein displayes disulfide reductase activity that is involved in the biogenesis of the plastid cytochrome b6f complex. Protein is located in the thylakoid membrane with the C-terminal hydrophilic portion, containing the thioredoxin like domain, extending into the thylakoid lumen.
Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana HCF164 protein sequence, UniProt: A0A178V430,, TAIR: AT4G37200
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized

 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Lyophilized antibody can be stored at -20°C for up to 3 years. 
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products collection of antibodies to electron transfer proteins
Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

28.6  | 26 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Aegilops tauschii, Anthurium amnicola, Cajanus cajan, Cephalotus follicularis, Cicer arietinum,  Corchorus olitorius, Cucumis melo, Dichanthelium oligosanthes, Glycine soja, Gossypium hirsutum, Medicago truncatula, Morus notabilis, Musa acuminata, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Oryza sativa subsp. japonica, Saccharum hybrid cultivar R570, Solanum chacoense , Theobroma cacao, Vigna radiata var. radiata, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

To be added when available, antibody available in September 2017.

Application example

western blot using anti-HCF164 antibodies

Arabidopsis thaliana Col0 thylakoids were extracted in isolation buffer (330 mm Sucrose, 25 mm HEPES-KOH, pH 7.4, 10 mm MgCl2, and 10 mm NaF) and detarud with Laemmli buffer at 65C for 10 min. Proteins were separed on 12 % SDS-PAGE and blotted  1 h on a PVDF membrane using semi-dry transfer. Blot was blocked with 8 % milk for 1 h at RT. Blot was incubated in the primary antibody at a dilution of 1: 3 000 overnight at 4°C with agitation in TBS-T with 1% milk. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:25 000 in  for 2h at RT with agitation. The blot was washed as above, incubated in ECL solution (GE Healthcare) for 5 min and developed on film with 1 min exposure.

Courtesy of Msc Lauri Nikkanen, University of Turku, Finland

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at