Thioredoxin-like protein HCF164, chloroplastic

AS16 4097 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Thioredoxin-like protein HCF164, chloroplastic

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana HCF164 protein sequence, UniProt: A0A178V430,, TAIR: AT4G37200

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 28,6 | 26 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Aegilops tauschii, Anthurium amnicola, Cajanus cajan, Cephalotus follicularis, Cicer arietinum, Corchorus olitorius, Cucumis melo, Dichanthelium oligosanthes, Glycine soja, Gossypium hirsutum, Medicago truncatula, Morus notabilis, Musa acuminata, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Oryza sativa subsp. japonica, Saccharum hybrid cultivar R570, Solanum chacoense , Theobroma cacao, Vigna radiata var. radiata, Zea mays
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-HCF164 antibodies

Arabidopsis thaliana Col0 thylakoids were extracted in isolation buffer (330 mm Sucrose, 25 mm HEPES-KOH, pH 7.4, 10 mm MgCl₂, and 10 mm NaF) and detarud with Laemmli buffer at 65C for 10 min. Proteins were separed on 12 % SDS-PAGE and blotted 1 h on a PVDF membrane using semi-dry transfer. Blot was blocked with 8 % milk for 1 h at RT. Blot was incubated in the primary antibody at a dilution of 1: 3 000 overnight at 4°C with agitation in TBS-T with 1% milk. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:25 000 in for 2h at RT with agitation. The blot was washed as above, incubated in chemiluminescent detection reagent for 5 min and developed on film with 1 min exposure.

Courtesy of Msc Lauri Nikkanen, University of Turku, Finland

Additional information

Background

Background Thioredoxin-like protein HCF164, chloroplastic is involved in maintaining cell redox homeostasis. The portein displayes disulfide reductase activity that is involved in the biogenesis of the plastid cytochrome b6f complex. Protein is located in the thylakoid membrane with the C-terminal hydrophilic portion, containing the thioredoxin like domain, extending into the thylakoid lumen.

Product citations

Picture (footer):	Application example Arabidopsis thaliana Col0 thylakoids were extracted in isolation buffer (330 mm Sucrose, 25 mm HEPES-KOH, pH 7.4, 10 mm MgCl₂, and 10 mm NaF) and detarud with Laemmli buffer at 65C for 10 min. Proteins were separed on 12 % SDS-PAGE and blotted 1 h on a PVDF membrane using semi-dry transfer. Blot was blocked with 8 % milk for 1 h at RT. Blot was incubated in the primary antibody at a dilution of 1: 3 000 overnight at 4°C with agitation in TBS-T with 1% milk. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:25 000 in for 2h at RT with agitation. The blot was washed as above, incubated in chemiluminescent detection reagent for 5 min and developed on film with 1 min exposure. Courtesy of Msc Lauri Nikkanen, University of Turku, Finland
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Aegilops tauschii, Anthurium amnicola, Cajanus cajan, Cephalotus follicularis, Cicer arietinum, Corchorus olitorius, Cucumis melo, Dichanthelium oligosanthes, Glycine soja, Gossypium hirsutum, Medicago truncatula, Morus notabilis, Musa acuminata, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Oryza sativa subsp. japonica, Saccharum hybrid cultivar R570, Solanum chacoense , Theobroma cacao, Vigna radiata var. radiata, Zea mays Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Thioredoxin-like protein HCF164, chloroplastic is involved in maintaining cell redox homeostasis. The portein displayes disulfide reductase activity that is involved in the biogenesis of the plastid cytochrome b6f complex. Protein is located in the thylakoid membrane with the C-terminal hydrophilic portion, containing the thioredoxin like domain, extending into the thylakoid lumen.
calculated \| apparent molecular mass [kDa]:	28,6 \| 26 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated peptide derived from Arabidopsis thaliana HCF164 protein sequence, UniProt: A0A178V430,, TAIR: AT4G37200
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Quantity:	50 µg
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)