Thioredoxin-like protein HCF164, chloroplastic
AS16 4097 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
28.6 | 26 kDa
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Aegilops tauschii, Anthurium amnicola, Cajanus cajan, Cephalotus follicularis, Cicer arietinum, Corchorus olitorius, Cucumis melo, Dichanthelium oligosanthes, Glycine soja, Gossypium hirsutum, Medicago truncatula, Morus notabilis, Musa acuminata, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Oryza sativa subsp. japonica, Saccharum hybrid cultivar R570, Solanum chacoense , Theobroma cacao, Vigna radiata var. radiata, Zea mays|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
To be added when available, antibody available in September 2017.
Arabidopsis thaliana Col0 thylakoids were extracted in isolation buffer (330 mm Sucrose, 25 mm HEPES-KOH, pH 7.4, 10 mm MgCl2, and 10 mm NaF) and detarud with Laemmli buffer at 65C for 10 min. Proteins were separed on 12 % SDS-PAGE and blotted 1 h on a PVDF membrane using semi-dry transfer. Blot was blocked with 8 % milk for 1 h at RT. Blot was incubated in the primary antibody at a dilution of 1: 3 000 overnight at 4°C with agitation in TBS-T with 1% milk. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 from Agrisera) diluted to 1:25 000 in for 2h at RT with agitation. The blot was washed as above, incubated in ECL solution (GE Healthcare) for 5 min and developed on film with 1 min exposure.
Courtesy of Msc Lauri Nikkanen, University of Turku, Finland
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