HDT3 | Histone deacetylase HDT3
AS16 3968 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Nuclei were isolated from Arabidopsis thaliana leaves and resuspended with SDS loading buffer and then denatured at 95oC for 10 min. Proteins were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry or tank transfer. Blots were blocked with 3% milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at 1:4 000 dilution for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBST at RT with agitation. Blot was incubated in radish peroxidase conjugated goat anti-rabbit IgG (AS09 602, Agrisera) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with chemiluminescent detection reagent. Exposure time was 100 seconds.
Note: increasing blocking to 10% milk may reduce background signal.
Courtesy of Dr. Xiangsong Cheng, Wisconsin Institute for Discovery, USA
HDT3 is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. This protein is also involved in the modulation of abscisic acid and stress-responsive genes.Synonymes:HD-tuins protein 3, Histone deacetylase 2c
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