HDT3 | Histone deacetylase HDT3
AS16 3968 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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|Recommended dilution||1 : 4000-1 : 8000 (WB)|
|Expected | apparent MW||
31.8 | 40 kDa
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Noccaea caerulescens|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
to be added when available, antibody released in October 2017
Nuclei were isolated from Arabidopsis thaliana leaves and resuspended with SDS loading buffer and then denatured at 95oC for 10 min. Proteins were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry or tank transfer. Blots were blocked with 3% milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at 1:4 000 dilution for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBST at RT with agitation. Blot was incubated in radish peroxidase conjugated goat anti-rabbit IgG (AS09 602, Agrisera) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Clarity™ Western ECL Substrate (170-5609, BIO-RAD). Exposure time was 100 seconds.
Note: increasing blocking to 10% milk may reduce background signal.
Courtesy of Dr. Xiangsong Cheng, Wisconsin Institute for Discovery, USA
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