showForm Anti-HDT3 | histone deacetylase ANTIBODY

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HDT3 | Histone deacetylase HDT3

AS16 3968 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

HDT3 | Histone deacetylase HDT3 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Nuclear signaling at Agrisera AB (Antibodies for research) (AS16 3968)

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Product Information

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana HDT3, UniProt: Q9LZR5, TAIR: At5g03740

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 4000-1 : 8000 (WB)
Expected | apparent MW

31.8 | 40 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Noccaea caerulescens
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

 western blot using anti-HDT3 antibodies
Nuclei were isolated from Arabidopsis thaliana leaves and resuspended with SDS loading buffer and then denatured at 95oC for 10 min. Proteins were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry or tank transfer. Blots were blocked with 3% milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at 1:4 000 dilution for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBST at RT with agitation. Blot was incubated in radish peroxidase conjugated goat anti-rabbit IgG (AS09 602, Agrisera) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with Clarity™ Western ECL Substrate (170-5609, BIO-RAD). Exposure time was 100 seconds.
Note: increasing blocking to 10% milk may reduce background signal.

Courtesy of Dr. Xiangsong Cheng, Wisconsin Institute for Discovery, USA

Additional information

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Background

Background

HDT3 is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. This protein is also involved in the modulation of abscisic acid and stress-responsive genes.Synonymes:HD-tuins protein 3, Histone deacetylase 2c

Product citations

Selected references Park et. al (2018). Epigenetic switch from repressive to permissive chromatin in response to cold stress. Proc Natl Acad Sci U S A. 2018 Jun 5;115(23):E5400-E5409. doi: 10.1073/pnas.1721241115.