HRP | Horseradish peroxidase

AS16 3951 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. rusticana, A. thaliana, C. elegans

Product Information

Immunogen Native peroxidase isolated and purified from horseradish,

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 1000-1 : 5000 (WB)

Expected | apparent MW 38,6 kDa

Reactivity

Confirmed reactivity Armoracia rusticana, Arabidopsis thaliana, Caenorhabditis elegans

Predicted reactivity
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example
Western blot using anti-HRP antibody

Proteins were isolated from poplar T89, barley, Arabidopsis thaliana, horseradish root mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 45 µg of total proteins and 45-7.5 µg of HRP were loaded into each lane and separated on 12% SDS-PAGE, and then blotted overnight onto PVDF membrane. Membranes were blocked with milk powder for 2 h and then incubated in the primary antibody solution overnight, which was then decanted and the membrane was washed 3 times for 5 min in TBST. Membrane was incubated at RT for 1 hour in 1:10 000 goat anti-Rabbit secondary antibody AS09 602, followed by washing steps as above. Membrane was developed for 2 min with chemiluminescent detection reagent according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 10 s increment time for around 100 s.

Additional information

Background

Horseradish peroxidase (HRP) is a secretory plant peroxidase that catalyzes the oxidation of small aromatic substrates, such as plant hormone and lignin precursors by hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress.