HSP16.6 | Class I heat shock protein 16.6 (cytosolic)

AS08 286 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 6803

Product Information

Immunogen

Recombinant protein. Synechocystis PCC 6803 Hsp16.6 CI (class one) UniProt: P72977

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW

16.6 kDa

Reactivity

Confirmed reactivity Synechocystis sp. PCC 6803

Predicted reactivity Synechococcus sp., Thermosynechococcus sp.

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

Western blot using anti-Hsp16.6 antibodies

Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000).

Additional information

This product can be sold containing proclin if requested

On a gradient gel Hsp16.6 runs closer to 11 kDa.

Related products

AS08 350 | Anti-DnaK2 | heat shock protein 70-2 (HSP70-2), rabbit antibodies

AS08 284 | Anti-HSP17.6 | cytosolic class I heat shock protein 17.6, chicken antibodies

AS07 254 | Anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibodies

AS07 255 | Anti-HSP17.7 | cytosolic class II heat shock protein 17.7, rabbit antibodies

collection of antibodies to heat shock proteins

Background

Hsp16.6 belongs to a family of class I of a small heat shock proteins. They are induced once a cell is stressed by an increased temperature. Synechocystis Hsp16.6 is involved in the development of thermotolerance and thylakoid stability. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.

Product citations

Selected references Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.