HSP16,6 | Class I heat shock protein 16,6 (cytosolic)

AS08 286 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 6803

HSP16,6 | Class I heat shock protein 16,6 (cytosolic)

Product Information

Immunogen

Recombinant protein. Synechocystis PCC 6803 Hsp16.6 CI (class one) UniProt: P72977

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW 16,6 kDa

Reactivity

Confirmed reactivity Synechocystis sp. PCC 6803

Predicted reactivity Synechococcus sp., Thermosynechococcus sp.
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

Western blot using anti-Hsp16.6 antibodies

Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000).

Reactant: Synechocystis

Application: Western Blotting

Pudmed ID: 24476911

Journal: Nucleic Acids Res

Figure Number: 3B

Published Date: 2014-04-01

First Author: Gunnelius, L., Hakkila, K., et al.

Impact Factor: 16.476

Open Publication

Upregulated and downregulated genes in ?rpoZ. (A) The 187 genes upregulated at least 2-fold, and the 212 genes downregulated to one half or less were arranged to gene categories according to Cyanobase. (B) Total proteins were isolated and the amounts of AtpE, RbcL, NdhJ, NdhK, NdhD3, HspA, Flv3 and Flv2 proteins were detected by western blotting.

Additional information

This product can be sold containing proclin if requested

On a gradient gel Hsp16,6 runs closer to 11 kDa

Background

Hsp16.6 belongs to a family of class I of a small heat shock proteins. They are induced once a cell is stressed by an increased temperature. Synechocystis Hsp16.6 is involved in the development of thermotolerance and thylakoid stability. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.

Product citations

Selected references Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.

Confirmed reactivity:	Synechocystis sp. PCC 6803
predicted reactivity:	Synechococcus sp., Thermosynechococcus sp. Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
background:	Hsp16.6 belongs to a family of class I of a small heat shock proteins. They are induced once a cell is stressed by an increased temperature. Synechocystis Hsp16.6 is involved in the development of thermotolerance and thylakoid stability. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
additional information:	This product can be sold containing proclin if requested
additional information (application):	On a gradient gel Hsp16,6 runs closer to 11 kDa
All references:	Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.
calculated \| apparent molecular mass [kDa]:	16,6 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant protein. Synechocystis PCC 6803 Hsp16.6 CI (class one) UniProt: P72977
Purity:	Serum
Quantity:	100 µl
recommended dilution:	1 : 5000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
More images:	Reactant: Synechocystis Application: Western Blotting Pudmed ID: 24476911 Journal: Nucleic Acids Res Figure Number: 3B Published Date: 2014-04-01 First Author: Gunnelius, L., Hakkila, K., et al. Impact Factor: 16.476 Open Publication Upregulated and downregulated genes in ?rpoZ. (A) The 187 genes upregulated at least 2-fold, and the 212 genes downregulated to one half or less were arranged to gene categories according to Cyanobase. (B) Total proteins were isolated and the amounts of AtpE, RbcL, NdhJ, NdhK, NdhD3, HspA, Flv3 and Flv2 proteins were detected by western blotting.
Picture (footer):	Application example Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000).