HSP16,6 | Class I heat shock protein 16,6 (cytosolic)
AS08 286 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 6803
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Recombinant protein. Synechocystis PCC 6803 Hsp16.6 CI (class one) UniProt: P72977
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Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000).
Application: Western Blotting
Pudmed ID: 24476911
Journal: Nucleic Acids Res
Figure Number: 3B
Published Date: 2014-04-01
First Author: Gunnelius, L., Hakkila, K., et al.
Impact Factor: 16.476Open Publication
Upregulated and downregulated genes in ?rpoZ. (A) The 187 genes upregulated at least 2-fold, and the 212 genes downregulated to one half or less were arranged to gene categories according to Cyanobase. (B) Total proteins were isolated and the amounts of AtpE, RbcL, NdhJ, NdhK, NdhD3, HspA, Flv3 and Flv2 proteins were detected by western blotting.
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Hsp16.6 belongs to a family of class I of a small heat shock proteins. They are induced once a cell is stressed by an increased temperature. Synechocystis Hsp16.6 is involved in the development of thermotolerance and thylakoid stability. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
Related products: HSP16,6 | Class I heat shock protein 16,6 (cytosolic)
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