HSP17,6 | Cytosolic class I heat shock protein 17,6 (chicken antibody)
AS08 284 | Clonality: Polyclonal | Host: Hen | Reactivity: Arabidopsis thaliana
|Data sheet||Product citations||Protocols||Add review|
10 µg of total protein from (C) Arabidopsis thaliana WT, non-stressed, (HS) Arabidopsis thaliana WT stressed at 38ºC were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000).
Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 26019256
Journal: J Exp Bot
Figure Number: E
Published Date: 2015-08-01
First Author: Mao, Z. & Sun, W.
Impact Factor: 6.088Open Publication
Identification of tip3;1 and tip3;2 T-DNA insertion mutants and three TIP3;1-RNAi transgenic lines (TIP3;1-RNAi/tip3;2) in the tip3;2 mutant background. (A) Schematic representation of the tip3;1 and tip3;2 T-DNA insertion mutant lines. A triangle indicates the position of the T-DNA insertion, and the arrow indicates its orientation. The genomic sequences corresponding to the coding region (black boxes), untranslated region (grey boxes), and introns (black lines) are indicated. The positions of the primers (31LP, 31RP, 32LP, and 32RP) used for PCR analysis of the tip3;1 and tip3;2 T-DNA insertion mutants, respectively, are also indicated. (B) PCR analysis of genomic DNA of Col, tip3;1, tip3;2, and tip3;1/tip3;2. LP, left primer; RP, right primer; LB, T-DNA left border primer. (C) Schematic representation of the construct used for the suppression of TIP3;1 in Arabidopsis seeds. RNAi technology was used with a segment of the TIP3;1 gene driven by the seed-specific TIP3;1 promoter. (D) qRT-PCR analysis of TIP3;1, TIP3;2, and ACT7 transcript abundance in mature seeds of Col, mutants, and RNAi lines. PP2A was used as the endogenous control, and the transcript abundance of TIP3;1, TIP3;2, and ACT7 was quantified by comparisons with that of PP2A. Values are means ąSD, n=3. (E) Immunoblot analysis of TIP3s in mature seeds from Col, tip3 mutants, and three TIP3;1-RNAi/tip3;2 transgenic lines (R3, R7, and R8). HSP17.6, which is expressed in mature seeds, was used as a loading control. (This figure is available in colour at JXB online.)
Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.
Related products: HSP17,6 | Cytosolic class I heat shock protein 17,6 (chicken antibody)
This product can b...