HSP17,7 | Cytosolic class II heat shock protein 17,7

AS07 255 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. tequiliana var. Weber, C. sativus, S. vulgaris, P. ternata

HSP17,7 | Cytosolic class II heat shock protein 17,7 in the group Antibodies Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS07 255)
HSP17,7 | Cytosolic class II heat shock protein 17,7


Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


full length recombinant protein produced in E. coli and purified by conventional methods (no affinity tag). Arabidopsis thaliana Hsp17.7 CII (class two), UniProt: O81822, TAIR: At5g12030

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 17,7 kDa


Confirmed reactivity Arabidopsis thaliana, Agave tequiliana var. Weber, Cucumis sativus, Medicago sativa, Pinellia ternata, Silene vulgaris
Predicted reactivity Dicots
Species of your interest not listed? Contact us
Not reactive in

Oryza sativa

Application examples

Application examples

Application example


western blot using anti-HSP17.7 antibodies

15 µg of total protein from (HS) heat shocked (38ºC/2h) Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.7 were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000). Development was done using chemiluminescent detection reagent,  according to the recommendations of the manufacturers.
Image was acquired with a 10 sec exposure time on X-ray film (Kodak). Band at ~38 kDa represents and SDS resistant dimer.

Additional information

Related products

Related products

AS07 254 | Anti-HSP17.6 | cytosolic class I heat shock protein 17.6, rabbit antibodies

AS08 284 | Anti-HSP17.6 | cytosolic class I heat shock protein 17.6 chicken antibodies

collection of antibodies to plant heat shock proteins



Hsp17.7 belongs to a family of class II of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

Product citations

Selected references Cha et al. (2020). Humic acid enhances heat stress tolerance via transcriptional activation of Heat-Shock Proteins in Arabidopsis. Sci Rep. 2020 Sep 14;10(1):15042.doi: 10.1038/s41598-020-71701-8.
McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263
Fu et al. (2019). Increased fes1a thermotolerance is induced by BAG6 knockout. Plant Mol Biol. 2019 Feb 22. doi: 10.1007/s11103-019-00844-8.
Korotaeva et al. (2018). Effect of Heat Hardening on Expression of Genes phb3 and phb4 and Accumulation of Phb Proteins in Green Leaves of Arabidopsis thaliana. Russian Journal of Plant Physiology, 65(5), 688-696, 2018.
McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1?12.
Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.
Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.
Luján et al. (2009). Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 32(12):1791-803.

Related products: HSP17,7 | Cytosolic class II heat shock protein 17,7

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €
AS08 286  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactiv...
381 €
AS07 254 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. tequilana, B. juncea...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items