HSP17.7 | Cytosolic class II heat shock protein 17.7
AS07 255 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. tequiliana var. Weber, C. sativus, S. vulgaris, P. ternata
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15 µg of total protein from (HS) heat shocked (38ºC/2h) Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.7 were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000). Development was done using chemiluminescent detection reagent, according to the recommendations of the manufacturers.
Image was acquired with a 10 sec exposure time on X-ray film (Kodak). Band at ~38 kDa represents and SDS resistant dimer.
Hsp17.7 belongs to a family of class II of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.
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Luján et al. (2009). Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 32(12):1791-803.