HSP18.5 | class IV heat shock protein

18.5 kDa

As hsp18.5 is a low abundancy protein, please, make sure that the plants are heated to the right temperature. Normally I heat stress the seedling on a sealed agar plate for 2 hours. This assures that the humidity around the plant is very high. Low humidity can allow the plant to cool down through transpiration.  If a plant is in soil you can keep the leaf or even a whole plant over a wet filter paper and seal the plate very well during the treatment. Heat stressing plants in microphage tubes does not work that well.

Hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38ºC for 3 hours at high humidity.

Please, note that longer transfer time might result in losing a signal for this protein.

AS08 286 | Anti-HSP16.6 | cytosolic class I heat shock protein 16.6, rabbit antibodies for cyanobacterial HSP16.6
AS07 254 | Anti-HSP17.6 | cytosolic class I heat shock protein 17.6, rabbit antibodies
AS08 284 | Anti-HSP17.6 | cytosolic class I heat shock protein 17.6, hen antibodies
AS07 255 | Anti-HSP17.7 | cytosolic class II heat shock protein 17.7, rabbit antibodies
AS08 285 | Anti-HSP21 | chloroplastic heat shock protein, rabbit antibodies

Plant protein extraction buffer

HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family and is involved in protein complex oligomerization and protein folding. Alternative names: AtHsp18.5, 18.5 kDa heat shock protein.