HSP18.5 | class IV heat shock protein

AS11 1628 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Hordeum vulgare

Product Information

Immunogen Recombinat Arabidopsis thaliana HSP18.5, expressed in E.coli, UniProt: O64564, TAIR: AT2G19310

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

18.5 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare

Predicted reactivity Glycne max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, Zea mays
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

As hsp18.5 is a low abundancy protein, please, make sure that the plants are heated to the right temperature. Normally I heat stress the seedling on a sealed agar plate for 2 hours. This assures that the humidity around the plant is very high. Low humidity can allow the plant to cool down through transpiration. If a plant is in soil you can keep the leaf or even a whole plant over a wet filter paper and seal the plate very well during the treatment. Heat stressing plants in microphage tubes does not work that well.

Hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38ºC for 3 hours at high humidity.

Please, note that longer transfer time might result in losing a signal for this protein.

Related products

Plant protein extraction buffer

Background

HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family and is involved in protein complex oligomerization and protein folding. Alternative names: AtHsp18.5, 18.5 kDa heat shock protein.

Product citations

Selected references Sadura et al. (2020). HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures . Int J Mol Sci . 2020 Mar 10;21(5):1889.doi: 10.3390/ijms21051889.