HSP18,5 | class IV heat shock protein

AS11 1628   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana, Hordeum vulgare

HSP18,5 | class IV heat shock protein in the group Antibodies Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS11 1628)
HSP18,5 | class IV heat shock protein


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Product Information

Immunogen Recombinat Arabidopsis thaliana HSP18.5, expressed in E.coli,  UniProt: O64564, TAIR: AT2G19310
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 18,5 kDa


Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare
Predicted reactivity Glycne max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, Zea mays
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information As hsp18,5 is a low abundancy protein, please, make sure that the plants are heated to the right temperature, Normally I heat stress the seedling on a sealed agar plate for 2 hours, This assures that the humidity around the plant is very high, Low humidity can allow the plant to cool down through transpiration, If a plant is in soil you can keep the leaf or even a whole plant over a wet filter paper and seal the plate very well during the treatment, Heat stressing plants in microphage tubes does not work that well

Hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38ºC for 3 hours at high humidity.

Please, note that longer transfer time might result in losing a signal for this protein.

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Plant protein extraction buffer



HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family and is involved in protein complex oligomerization and protein folding. Alternative names: AtHsp18.5, 18.5 kDa heat shock protein.

Product citations

Selected references Sadura et al. (2020). HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures . Int J Mol Sci . 2020 Mar 10;21(5):1889.doi: 10.3390/ijms21051889.

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