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HSP21 | Chloroplastic heat shock protein

AS08 285 |Clonality: Polyclonal | Host: Rabbit | Reactivity: A. stolonifera cv. ‘Penncross’, A.thaliana, N. tabacum

HSP21 | Chloroplastic heat shock protein in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS08 285)

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Product Information

Immunogen

Recombinant Hsp21 protein derived from the sequence of Arabidopsis thaliana hsp21 UniProt: P31170, TAIR:At4g27670

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW

25 | 21 kDa

Reactivity

Confirmed reactivity Agostis stolonifera cv. ‘Penncross’, Arabidopsis thaliana, Nicotiana tabacum
Not reactive in

Chlamydomonas reinhardtii

Application examples

Additional information

Additional information

Recommended load per well 20 µg of total protein

This product can be sold containing proClin if requested.

Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C.

Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.

Related products

Related products

AS08 284 | Anti-HSP17.6 | cytosolic class I heat shock protein 17.6 chicken antibodies

AS07 254 | Anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibodies

AS07 255 | Anti-HSP17.7 | cytosolic class II heat shock protein 17.7, rabbit antibodies

collection of antibodies to plant heat shock proteins

Background

Background

HSP21 is a chloroplast-localized small heat shock protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.

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Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items