HTA9 | Probable histone H2A variant 3

PRODUCT INFORMATION IN PDF

product information Background Variant histone H2A may replace conventional H2A in a subset of nucleosomes. Synonymes: H2A.F/Z 3 Immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana HTA9 UniProt: Q9C944, TAIR: At1g52740 Host Rabbit Clonality Polyclonal Clone Purity Affinity purified serum in PBS, pH 7.4 Format Lyophilized Quantity 50 µg Reconstitution For reconstitution add 50 µl of sterile water. Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications Western blot (WB) Related products AS11 1801 | anti-H1 | Histone H1, rabbit antibodies AS10 710 | anti-H3 | Histone H3, rabbit antibody AS10 710A | anti-H3 | Histone H3 (affinity purified), rabbit antibodies for ChIP AS11 1753 | anti-HDT3 | Histone deacetylase, rabbit antibody collection of antibodies to DNA/RNA/cell cycle Plant protein extraction buffer Secondary antibodies Additional information

application information
Recommended dilution		1 : 1000 (WB)
Expected | apparent MW		14.2 kDa (Arabidopsis thaliana)
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		To be added when available, antibody released in June 2016.

Application example



1) mutant hta9, hta11 about 1 ug protein; 2) mutant hta9, hta11 about 2 ug protein; 3) wild-type about 2-3 ug protein; 4) wild-type about 4-5 ug protein from Arabidopsis thaliana. 
Protein from Arabidopsis histone preparation (acid extracted, precipitated with acetone and resuspended in urea). Denatured with SDS-PAGE buffer at 90C for 2 min, separated on 15% SDS-PAGE and blotted 1h to PVDF tank transfer. Blots were blocked with PBS + 3% BSA overnight at 4C with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 in PBS + 3% BSA for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed once with PBS-T for 5 min. Blot was incubated in secondary antibody PBS + 3% BSA (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 for 1h at RT with agitation. The blot was washed 3-4 times with PBS-T for 5 min. The blot was developed with Immobilion (Millipore) and exposed on an X-ray film for 1 min.

---

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com