HTA9 | Probable histone H2A variant 3

AS10 718 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

HTA9 | Probable histone H2A variant 3 in the group Plant/Algal Antibodies / DNA/RNA/Cell Cycle / Nuclear signaling at Agrisera AB (Antibodies for research) (AS10 718)


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product information

Variant histone H2A may replace conventional H2A in a subset of nucleosomes. Synonymes: H2A.F/Z 3


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana HTA9 UniProt: Q9C944, TAIR: At1g52740

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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AS11 1753 | anti-HDT3 | Histone deacetylase, rabbit antibody

collection of antibodies to DNA/RNA/cell cycle

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

14.2 kDa (Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

To be added when available, antibody released in June 2016.

Application example

western blot using anti-HTA9 antibodies

1) mutant hta9, hta11 about 1 ug protein; 2) mutant hta9, hta11 about 2 ug protein; 3) wild-type about 2-3 ug protein; 4) wild-type about 4-5 ug protein from Arabidopsis thaliana
Protein from Arabidopsis histone preparation (acid extracted, precipitated with acetone and resuspended in urea). Denatured with SDS-PAGE buffer at 90C for 2 min, separated on 15% SDS-PAGE and blotted 1h to PVDF tank transfer. Blots were blocked with PBS + 3% BSA overnight at 4C with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 in PBS + 3% BSA for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed once with PBS-T for 5 min. Blot was incubated in secondary antibody PBS + 3% BSA (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 for 1h at RT with agitation. The blot was washed 3-4 times with PBS-T for 5 min. The blot was developed with Immobilion (Millipore) and exposed on an X-ray film for 1 min.

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