IAA | indole-3-acetic acid (N1) (for immunolocalization)

AS09 421 | Clonality: Polyclonal | Host: Rabbit | Reactivity:indole-3-acetic acid (N1) tested so far in: Eucalyptus globulus, Juncus prismatocarpus subsp. leschenaultii Kirschner (J. prismatocarpus), Juncus wallichianus Laharpe (J. wallichianus), Pinus radiata, Vitis vinifera

IAA | indole-3-acetic acid (N1) (for immunolocalization)

Product Information

Immunogen

BSA-conjugated, via N1 group of indole, indole-3-acetic acid (N1)

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein G purified in PBS.

Format Liquid

Quantity 200 µg

Storage Freeze upon arrival and store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunolocalization (IL)

Recommended dilution Specific information about dilution is going to be included on the vial

Reactivity

Confirmed reactivity indole-3-acetic acid (N1) tested so far in: Eucalyptus globulus, Juncus prismatocarpus subsp. leschenaultii Kirschner (J. prismatocarpus), Juncus wallichianus Laharpe (J. wallichianus), Pinus radiata, Vitis vinifera

Predicted reactivity Indole-3-acetic acid (N1)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information IAA | indole-3-acetic acid (N1) (for immunolocalization)

Antibody can be used for direct detection of free IAA (see reference below)

Antibody is provided in 50% glycerol. For larger quantity (1 mg)- please inquire.

Steedman's wax embedding technique is recommended to be used with this antibody. The most critical issue is to keep the temperature below 37°C during the whole embedding procedure. More information can be found in Vitha et al. (2000).

Background

Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.

Product citations

Selected references Nukazuka et al. (2021). A Role for Auxin in Triggering Lamina Outgrowth of Unifacial Leaves. Plant Physiol. 2021 Feb 23:kiab087. doi: 10.1093/plphys/kiab087. Epub ahead of print. PMID: 33620494.
Dinis et al. (2018). Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress. J Plant Physiol. 2018 Jan;220:181-192. doi: 10.1016/j.jplph.2017.11.007.
Escandón et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.
Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56?66.
De Diego et al. (2013).Immunolocalization ofIAA andABA inroots andneedles ofradiatapine (Pinusradiata) duringdrought andrewatering. Tree Physiol., May; 33(5):537-549.

Confirmed reactivity:	indole-3-acetic acid (N1) tested so far in: Eucalyptus globulus, Juncus prismatocarpus subsp. leschenaultii Kirschner (J. prismatocarpus), Juncus wallichianus Laharpe (J. wallichianus), Pinus radiata, Vitis vinifera
predicted reactivity:	Indole-3-acetic acid (N1)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	IAA \| indole-3-acetic acid (N1) (for immunolocalization)
additional information (application):	Antibody can be used for direct detection of free IAA (see reference below) Antibody is provided in 50% glycerol. For larger quantity (1 mg)- please inquire. Steedman's wax embedding technique is recommended to be used with this antibody. The most critical issue is to keep the temperature below 37°C during the whole embedding procedure. More information can be found in Vitha et al. (2000).
background:	Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
All references:	Nukazuka et al. (2021). A Role for Auxin in Triggering Lamina Outgrowth of Unifacial Leaves. Plant Physiol. 2021 Feb 23:kiab087. doi: 10.1093/plphys/kiab087. Epub ahead of print. PMID: 33620494. Dinis et al. (2018). Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress. J Plant Physiol. 2018 Jan;220:181-192. doi: 10.1016/j.jplph.2017.11.007. Escandón et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12. Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56?66. De Diego et al. (2013).Immunolocalization ofIAA andABA inroots andneedles ofradiatapine (Pinusradiata) duringdrought andrewatering. Tree Physiol., May; 33(5):537-549.
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	BSA-conjugated, via N1 group of indole, indole-3-acetic acid (N1)
Purity:	Total IgG. Protein G purified in PBS.
Quantity:	200 µg
recommended dilution:	Specific information about dilution is going to be included on the vial
storage:	Freeze upon arrival and store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunolocalization (IL)

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