IAA | Indole 3 acetic acid
AS06 193 | Clonality: Polyclonal | Host: Rabbit | Reactivity: IAA | Indole 3 acetic acid
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Product Information
BSA-conjugated synthetic indole 3 acetic acid
Reactivity
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Application examples
Application example
4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS11 1815) as secondary antibody diluted in 1: 3000 (shown in red color) were used.
Courtesy Dr. Taras Pasternak, Freiburg University, Germany.
Reactant: Arabidopsis thaliana (Thale cress)
Application: Immunohistochemistry-immunofluorescence
Pudmed ID: 26516341
Journal: Plant Methods
Figure Number: 8A
Published Date: 2015-10-31
First Author: Pasternak, T., Tietz, O., et al.
Impact Factor: 5.139
Open PublicationAuxin immunolocalisation in Arabidopsis roots. Four days old Arabidopsis seedlings were treated with 1 ĩM 1-N-Naphthylphthalamic acid (NPA) for 24 h to enhance accumulation of auxin in roots. Seedlings were fixed for 20 min in 4 % EDAC in 1Ũ MTSB, and next 30 min in 4 % EDAC+ 2 % Formaldehyde. Anti-indole 3 acetic acid (IAA) rabbit primary antibody (Agrisera, AS06 193) diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLightŪ 549 Conjugate (AS09 633) as secondary antibody diluted in 1:3000 (shown in red color) were used. Scale bar 20 ĩm
Additional information
For detailed immunolocalization protocol check here.
Quantification of IAA in ELISA using this antibody is described in Bianco and Defez (2009).
Whole-mount immunolocalization is described in Lu et al. (2015).
Background
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
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