IAA | Indole 3 acetic acid

AS06 193 | Clonality: Polyclonal | Host: Rabbit | Reactivity: IAA | Indole 3 acetic acid

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Product Information

Immunogen

BSA-conjugated synthetic indole 3 acetic acid

Host Rabbit

Clonality Polyclonal

Purity Total IgG

Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7,35

Quantity 1 mg

Reconstitution For reconstitution add 50 µl of sterile water and 50 µl of glycerol

Storage Store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

Tested applications ELISA (ELISA), Immunolocalization (IL)

Recommended dilution 1 : 5000-1 : 10 000 (ELISA), 1 : 100-1 :600 (IL)

Reactivity

Confirmed reactivity Arabidoppsis thaliana, Drosera capensis, Euphorbia pulcherrima, Medicago sativa (nodules)

Predicted reactivity Dicots
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

immunolocalization of auxin in plant tissue

4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS11 1815) as secondary antibody diluted in 1: 3000 (shown in red color) were used.

Courtesy Dr. Taras Pasternak, Freiburg University, Germany.

Reactant: Arabidopsis thaliana (Thale cress)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 26516341

Journal: Plant Methods

Figure Number: 8A

Published Date: 2015-10-31

First Author: Pasternak, T., Tietz, O., et al.

Impact Factor: 5.139

Open Publication

Auxin immunolocalisation in Arabidopsis roots. Four days old Arabidopsis seedlings were treated with 1 µM 1-N-Naphthylphthalamic acid (NPA) for 24 h to enhance accumulation of auxin in roots. Seedlings were fixed for 20 min in 4 % EDAC in 1× MTSB, and next 30 min in 4 % EDAC+ 2 % Formaldehyde. Anti-indole 3 acetic acid (IAA) rabbit primary antibody (Agrisera, AS06 193) diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS09 633) as secondary antibody diluted in 1:3000 (shown in red color) were used. Scale bar 20 µm

Additional information

Additional information Total IgG was purified by ammonium sulfate precipiation

For detailed immunolocalization protocol check here.

Quantification of IAA in ELISA using this antibody is described in Bianco and Defez (2009).

Whole-mount immunolocalization is described in Lu et al. (2015).

Background

Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.

Product citations

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