IAA | Indole 3 acetic acid

BSA-conjugated synthetic indole 3 acetic acid 

Application example

4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS11 1815) as secondary antibody diluted in 1: 3000 (shown in red color) were used.

Courtesy Dr. Taras Pasternak, Freiburg University, Germany.

For detailed immunolocalization protocol check here.

Quantification of IAA in ELISA using this antibody is described in Bianco and Defez (2009).

Whole-mount immunolocalization is described in Lu et al. (2015).

AS09 445 | Anti-indole- 3- acetic acid (C1') for immunolocalization, rabbit antibodies

AS09 420 | Anti-indole 3 acetic acid (C1') (for ELISA), rabbit antibodies

AS09 421 | Anti-indole 3 acetic acid (N1), rabbit antibodies

AS12 1865 | Anti-Ethylene insensitive 2, rabbit antibodies

collection of antibodies to plant hormones

Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in  in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.