Agrisera Super Deal

Primary/Secondary/ECL

Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)

- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright, for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

IAA | Indole 3 acetic acid

AS06 193 | Clonality: Polyclonal | Host: Rabbit

PRODUCT INFORMATION IN PDF

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product information
Background		Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
Immunogen		BSA-conjugated synthetic indole 3 acetic acid
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Total IgG
Format		Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity		100 µl
Reconstitution		For reconstitution add 50 µl of sterile water and 50 µl of glycerol.
Storage		Store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		ELISA (ELISA), Immunolocalization (IL)
Related products		AS09 445 \| anti-indole- 3- acetic acid (C1') for immunolocalization AS09 420 \| anti-indole 3 acetic acid (C1') (for ELISA) AS09 421 \| anti-indole 3 acetic acid (N1) AS12 1865 \| Ethylene insensitive 2 collection of antibodies to plant hormones
Additional information		Total IgG was purified by ammonium sulfate precipiation.

application information
Recommended dilution		1 : 5000-1 : 10 000 (ELISA), 1 : 100-1 :600 (IL)
Expected \| apparent MW
Confirmed reactivity		Arabidoppsis thaliana, Euphorbia pulcherrima, Medicago sativa (nodules)
Predicted reactivity		Dicots
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		For detailed immunolocalization protocol check here. Quantification of IAA in ELISA using this antibody is described in Bianco and Defez (2009). Whole-mount immunolocalization is described in Lu et al. (2015).
Selected references		Lu et al. (2015). OsPIN5b modulates rice plant architecture and yield by changing auxin homeostasis, transport and distribution. Plant J. 2015 Jul 25. doi: 10.1111/tpj.12939. Bianco and Defez (2009). Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain.J Exp. Bot. 60, No. 11: 3097-3107.

application example

immunolocalization of auxin in plant tissue

4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS11 1815) as secondary antibody diluted in 1: 3000 (shown in red color) were used.

Courtesy Dr. Taras Pasternak, Freiburg University, Germany.

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