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IAA | Indole 3 acetic acid

AS06 193  |  Clonality: Polyclonal  |  Host: Rabbit | Reactivity: IAA | Indole 3 acetic acid

Sold out. Other antibodies to IAA can be found here

IAA | Indole 3 acetic acid in the group Antibodies Plant/Algal  / Hormones / Auxins at Agrisera AB (Antibodies for research) (AS06 193)



DATA SHEET IN PDF

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Product Information

Immunogen

BSA-conjugated synthetic indole 3 acetic acid 

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7,35
Quantity 1 mg
Reconstitution For reconstitution add 50 ĩl of sterile water and 50 ĩl of glycerol
Storage Store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes
Tested applications ELISA (ELISA), Immunolocalization (IL)
Recommended dilution 1 : 5000-1 : 10 000 (ELISA), 1 : 100-1 :600 (IL)

Reactivity

Confirmed reactivity Arabidoppsis thaliana, Drosera capensis, Euphorbia pulcherrima, Medicago sativa (nodules)
Predicted reactivity Dicots
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

immunolocalization of auxin in plant tissue

4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS11 1815) as secondary antibody diluted in 1: 3000 (shown in red color) were used.

Courtesy Dr. Taras Pasternak, Freiburg University, Germany.

Reactant: Arabidopsis thaliana (Thale cress)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 26516341

Journal: Plant Methods

Figure Number: 8A

Published Date: 2015-10-31

First Author: Pasternak, T., Tietz, O., et al.

Impact Factor: 5.139

Open Publication

Auxin immunolocalisation in Arabidopsis roots. Four days old Arabidopsis seedlings were treated with 1 ĩM 1-N-Naphthylphthalamic acid (NPA) for 24 h to enhance accumulation of auxin in roots. Seedlings were fixed for 20 min in 4 % EDAC in 1Ũ MTSB, and next 30 min in 4 % EDAC+ 2 % Formaldehyde. Anti-indole 3 acetic acid (IAA) rabbit primary antibody (Agrisera, AS06 193) diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLightŪ 549 Conjugate (AS09 633) as secondary antibody diluted in 1:3000 (shown in red color) were used. Scale bar 20 ĩm

Additional information

Additional information Total IgG was purified by ammonium sulfate precipiation

For detailed immunolocalization protocol check here.

Quantification of IAA in ELISA using this antibody is described in Bianco and Defez (2009).

Whole-mount immunolocalization is described in Lu et al. (2015).

Related products

Related products

AS09 445 | Anti-indole- 3- acetic acid (C1') for immunolocalization, rabbit antibodies

AS09 420 | Anti-indole 3 acetic acid (C1') (for ELISA), rabbit antibodies

AS09 421 | Anti-indole 3 acetic acid (N1), rabbit antibodies

AS12 1865 | Anti-Ethylene insensitive 2, rabbit antibodies

collection of antibodies to plant hormones

Background

Background

Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in  in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.

Product citations

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