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Product Information
BSA-conjugated synthetic indole 3 acetic acid
Reactivity
Application examples
Application example
4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS11 1815) as secondary antibody diluted in 1: 3000 (shown in red color) were used.
Courtesy Dr. Taras Pasternak, Freiburg University, Germany.
Additional information
For detailed immunolocalization protocol check here.
Quantification of IAA in ELISA using this antibody is described in Bianco and Defez (2009).
Whole-mount immunolocalization is described in Lu et al. (2015).
Background
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
Product citations
Nishimura and Koshiba (2019). Immunolocalization of IAA Using an Anti-IAA-C-Antibody Raised Against Carboxyl-Linked IAA. Phototropism. Methods in Molecular Biology, vol 1924. Humana Press, New York, NY.
Lu et al. (2015). OsPIN5b modulates rice plant architecture and yield by changing auxin homeostasis, transport and distribution. Plant J. 2015 Jul 25. doi: 10.1111/tpj.12939.
Bianco and Defez (2009). Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain.J Exp. Bot. 60, No. 11: 3097-3107.
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