IAA | Indole 3 acetic acid
AS06 193 | Clonality: Polyclonal | Host: Rabbit | Reactivity: IAA | Indole 3 acetic acid
Sold out. Other antibodies to IAA can be found here.
|Data sheet||Product citations||Protocols||Add review|
BSA-conjugated synthetic indole 3 acetic acid
Species of your interest not listed? Contact us
4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS11 1815) as secondary antibody diluted in 1: 3000 (shown in red color) were used.
Courtesy Dr. Taras Pasternak, Freiburg University, Germany.
Reactant: Arabidopsis thaliana (Thale cress)
Pudmed ID: 26516341
Journal: Plant Methods
Figure Number: 8A
Published Date: 2015-10-31
First Author: Pasternak, T., Tietz, O., et al.
Impact Factor: 5.139Open Publication
Auxin immunolocalisation in Arabidopsis roots. Four days old Arabidopsis seedlings were treated with 1 ĩM 1-N-Naphthylphthalamic acid (NPA) for 24 h to enhance accumulation of auxin in roots. Seedlings were fixed for 20 min in 4 % EDAC in 1Ũ MTSB, and next 30 min in 4 % EDAC+ 2 % Formaldehyde. Anti-indole 3 acetic acid (IAA) rabbit primary antibody (Agrisera, AS06 193) diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLightŪ 549 Conjugate (AS09 633) as secondary antibody diluted in 1:3000 (shown in red color) were used. Scale bar 20 ĩm
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.