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IgG1 (Negative Control, clone VI-AP)

AS20 4450 | Clonality: Monoclonal  Host: Mouse | Reactivity: calf intestine alkaline phosphatase

Offered at a 20 % discount. Order today with a discount code: Block20

IgG1 (Negative Control, clone VI-AP) in the group Secondary Antibodies / Blocking/Control / Negative control at Agrisera AB (Antibodies for research) (AS20 4450)

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Product Information

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Affinity purified
Format Liquid in PBS pH 7.2, 1% BSA, 0.05% NaN3
Quantity 0.2 mg
Storage Stored at 2-8°C. Do not freeze.
Tested applications Direct Immunofluorescence (IF), Flow Cytometry (Flow cyt), Immunofluoresence (IF)

Reactivity

Confirmed reactivity Calf intestine alkaline phosphatase (no cross reaction with human proteins)

Application examples

Additional information

Additional information The clone VI-AP reacts with calf intestine alkaline phosphatase and does not show cross-reactivity with human proteins.

The sensitivity of VI-AP mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point and the detection threshold  In practice, 50 µl of leukocytes containing 10^7 cells/ml are stained with 20 µl mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold.

Enough for approximately 100 of tests.

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Background

Background

Mouse immunoglobulin molecules of IgG1 isotype this ready to use Negative Control reagent contains purified non-conjugated mouse immunoglobulin molecules of IgG1 isotype, which have been selected on the basis of their binding characteristics: no specific binding to human cell surface or intracellular antigens

Product citations

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