IgG1 (Negative Control, clone VI-AP)
Product no: AS20 4450
AS20 4450 | Clonality: Monoclonal Host: Mouse | Reactivity: calf intestine alkaline phosphatase
138
€
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3-6 business days
- Product Info
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Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: IgG1 immunoglobulin affinity purified in PBS pH 7.2. Contains 1% BSA, 0.05% sodium azide. Format: Liquid Quantity: 0.2 mg Storage: Stored at 2-8°C, do not freeze. Tested applications: Direct Immunofluorescence (IF), Flow Cytometry (Flow cyt), Immunofluoresence (IF) - Reactivity
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Confirmed reactivity: Calf intestine alkaline phosphatase (no cross reaction with human proteins) - Additional Information
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Additional information: The clone VI-AP reacts with calf intestine alkaline phosphatase and does not show cross-reactivity with human proteins.
The sensitivity of VI-AP mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point and the detection threshold In practice, 50 µl of leukocytes containing 10^7 cells/ml are stained with 20 µl mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold.Additional information (application): Enough for approximately 100 of tests - Background
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Background: Mouse immunoglobulin molecules of IgG1 isotype this ready to use Negative Control reagent contains purified non-conjugated mouse immunoglobulin molecules of IgG1 isotype, which have been selected on the basis of their binding characteristics: no specific binding to human cell surface or intracellular antigens
- Protocols
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Antibody Protocols
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