IgG1 (Negative Control, clone VI-AP)
AS20 4450 | Clonality: Monoclonal Host: Mouse | Reactivity: calf intestine alkaline phosphatase

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Product Information
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
IgG1 immunoglobulin affinity purified in PBS pH 7.2. Contains 1% BSA, 0.05% sodium azide.
Format
Liquid
Quantity
0,2 mg
Storage
Stored at 2-8°C, do not freeze.
Tested applications
Direct Immunofluorescence (IF), Flow Cytometry (Flow cyt), Immunofluoresence (IF)
Reactivity
Confirmed reactivity
Calf intestine alkaline phosphatase (no cross reaction with human proteins)
Application examples
Additional information
Additional information
The clone VI-AP reacts with calf intestine alkaline phosphatase and does not show cross-reactivity with human proteins.
The sensitivity of VI-AP mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point and the detection threshold In practice, 50 µl of leukocytes containing 10^7 cells/ml are stained with 20 µl mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold.
The sensitivity of VI-AP mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point and the detection threshold In practice, 50 µl of leukocytes containing 10^7 cells/ml are stained with 20 µl mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold.
Enough for approximately 100 of tests
Background
Background
Mouse immunoglobulin molecules of IgG1 isotype this ready to use Negative Control reagent contains purified non-conjugated mouse immunoglobulin molecules of IgG1 isotype, which have been selected on the basis of their binding characteristics: no specific binding to human cell surface or intracellular antigens
Product citations
Related products: IgG1 (Negative Control, clone VI-AP)
AS20 4452 | Clonality: Monoclonal | Host: Mouse | Reactivity: Mouse IgG1
158 €