IgG1 (Negative Control, clone VI-AP)

Product no: AS20 4450

AS20 4450 | Clonality: Monoclonal  Host: Mouse | Reactivity: calf intestine alkaline phosphatase

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Delivery:  3-6 business days
  • Product Info
  • Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: IgG1 immunoglobulin affinity purified in PBS pH 7.2. Contains 1% BSA, 0.05% sodium azide.
    Format: Liquid
    Quantity: 0.2 mg
    Storage: Stored at 2-8°C, do not freeze.
    Tested applications: Direct Immunofluorescence (IF), Flow Cytometry (Flow cyt), Immunofluoresence (IF)
  • Reactivity
  • Confirmed reactivity: Calf intestine alkaline phosphatase (no cross reaction with human proteins)
  • Additional Information
  • Additional information: The clone VI-AP reacts with calf intestine alkaline phosphatase and does not show cross-reactivity with human proteins.

    The sensitivity of VI-AP mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point and the detection threshold  In practice, 50 µl of leukocytes containing 10^7 cells/ml are stained with 20 µl mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold.
    Additional information (application): Enough for approximately 100 of tests
  • Background
  • Background:

    Mouse immunoglobulin molecules of IgG1 isotype this ready to use Negative Control reagent contains purified non-conjugated mouse immunoglobulin molecules of IgG1 isotype, which have been selected on the basis of their binding characteristics: no specific binding to human cell surface or intracellular antigens

  • Protocols
  • Antibody Protocols
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AS20 4452 | Clonality: Monoclonal | Host: Mouse | Reactivity: Mouse IgG1

167 €
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