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Mouse IgG1 (Negative Control, clone ZX3)

AS20 4452 | Clonality: Monoclonal | Host: Mouse | Reactivity: Mouse IgG1

Mouse IgG1 (Negative Control, clone ZX3) in the group Secondary Antibodies / Blocking/Control / Negative control at Agrisera AB (Antibodies for research) (AS20 4452)

DATA SHEET IN PDF

Qty: 
150
How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Protein A/G purified
Format Liquid in PBS with 0.08% sodium azide
Quantity 100 µg
Storage Stored at 4-8°C. Do not freeze.
Tested applications Flow Cytometry (Flowcyt)

Reactivity

Confirmed reactivity Mouse IgG1

Application examples

Additional information

Additional information PBMC: Add 10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. Whole blood: Add10 μl of MAB/100 μl of Whole Blood. Mix gently and 1/3incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Allophycocyanin: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions.

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Background

Background

Mouse IgG1 is a negative control for Immunofluorescence staining with  FITC, Biotin, PE and APC mouse monoclonal antibodies of the IgG1 subclass. Monitoring the level of non-specific binding of mouse monoclonal antibodies to human cell surgace antigens can be done using this product.

Product citations

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