Mouse IgG1 (Negative Control, clone ZX3)
Product no: AS20 4452
AS20 4452 | Clonality: Monoclonal | Host: Mouse | Reactivity: Mouse IgG1
167
€
Delivery:
3-6 business days
- Product Info
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Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: IgG1 immunoglobulin Protein A/G purified in PBS. Contains 0.08% sodium azide. Format: Liquid Quantity: 100 µg Storage: Stored at 2-8°C, do not freeze. Tested applications: Flow Cytometry (Flowcyt) - Reactivity
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Confirmed reactivity: Mouse IgG1 - Additional Information
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Additional information: PBMC: Add 10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. Whole blood: Add10 μl of MAB/100 μl of Whole Blood. Mix gently and 1/3incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Allophycocyanin: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions. - Background
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Background: Mouse IgG1 is a negative control for Immunofluorescence staining with FITC, Biotin, PE and APC mouse monoclonal antibodies of the IgG1 subclass. Monitoring the level of non-specific binding of mouse monoclonal antibodies to human cell surgace antigens can be done using this product.
- Protocols
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Antibody Protocols
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