IKP

Mouse IgG1 (Negative Control, clone ZX3)

Product no: AS20 4452

AS20 4452 | Clonality: Monoclonal | Host: Mouse | Reactivity: Mouse IgG1

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  • Product Info
  • Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: IgG1 immunoglobulin Protein A/G purified in PBS. Contains 0.08% sodium azide.
    Format: Liquid
    Quantity: 100 g
    Storage: Stored at 2-8C, do not freeze.
    Tested applications: Flow Cytometry (Flowcyt)
  • Reactivity
  • Confirmed reactivity: Mouse IgG1
  • Additional Information
  • Additional information: PBMC: Add 10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. Whole blood: Add10 μl of MAB/100 μl of Whole Blood. Mix gently and 1/3incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
    Allophycocyanin: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions.
  • Background
  • Background:

    Mouse IgG1 is a negative control for Immunofluorescence staining with  FITC, Biotin, PE and APC mouse monoclonal antibodies of the IgG1 subclass. Monitoring the level of non-specific binding of mouse monoclonal antibodies to human cell surgace antigens can be done using this product.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody Protocols
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