Mouse IgG1 (Negative Control, clone ZX3)

AS20 4452 | Clonality: Monoclonal | Host: Mouse | Reactivity: Mouse IgG1

Product Information

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity IgG1 immunoglobulin Protein A/G purified in PBS. Contains 0.08% sodium azide.

Format Liquid

Quantity 100 µg

Storage Stored at 2-8°C, do not freeze.

Tested applications Flow Cytometry (Flowcyt)

Reactivity

Confirmed reactivity Mouse IgG1

Application examples

Additional information

Additional information PBMC: Add 10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. Whole blood: Add10 μl of MAB/100 μl of Whole Blood. Mix gently and 1/3incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Allophycocyanin: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions.

Background

Mouse IgG1 is a negative control for Immunofluorescence staining with FITC, Biotin, PE and APC mouse monoclonal antibodies of the IgG1 subclass. Monitoring the level of non-specific binding of mouse monoclonal antibodies to human cell surgace antigens can be done using this product.

Product citations

Related products: Mouse IgG1 (Negative Control, clone ZX3)

IgG1 (Negative Control, clone VI-AP)

AS20 4450 | Clonality: Monoclonal Host: Mouse | Reactivity: calf intestine alkaline phosphat...

127 €

Clonality:	Monoclonal
Format:	Liquid
Host:	Mouse
Purity:	IgG1 immunoglobulin Protein A/G purified in PBS. Contains 0.08% sodium azide.
Quantity:	100 µg
storage:	Stored at 2-8°C, do not freeze.
Sub class:	IgG1
tested applications:	Flow Cytometry (Flowcyt)
background:	Mouse IgG1 is a negative control for Immunofluorescence staining with FITC, Biotin, PE and APC mouse monoclonal antibodies of the IgG1 subclass. Monitoring the level of non-specific binding of mouse monoclonal antibodies to human cell surgace antigens can be done using this product.
additional information:	PBMC: Add 10 µl of MAB/10^6 PBMC in100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze. Whole blood: Add10 μl of MAB/100 μl of Whole Blood. Mix gently and 1/3incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Allophycocyanin: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions.
Confirmed reactivity:	Mouse IgG1