KrP | Kelch repeat-containing protein / galactose oxidase (At3g07720)

Product no: AS14 2764

AS14 2764  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: Recombiant KrP derived from Arabidopsis thaliana sequence, UniProt: Q9S7W4, TAIR: At3g07720
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW: 35,7 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example
    Western blot with anti-KrP antibody

    15 µg of total protein from Arabidopsis thaliana Col-0 root or shoot grown on plates with (+) or without iron (-) extracted and denatured with 2X Laemmli buffer (100 mM Tris pH 6.8; 12 % glycerol, 0.01 % bromophenolblue, 2 % β-mercaptoethanol, 4 % SDS) at 95°C for 5 min followed by 5 min on ice. Samples were separated on 4-20 % Mini-PROTEAN TGX Stain-Free gel and blotted 60 min to nitrocelulose membrane using wet transfer. Blots were blocked with 5% milk powder dissolved in TBST for 30 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 for 60 min at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBST buffer at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:4000 in for 60 min at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent, using the FluorChem Q (protein simple) detection system. Exposure time was 3.2 seconds.

  • Additional Information
  • Additional information: KrP (NSL1) and NSP5 (nitrile-specifier protein 5) share 53% of amino acid sequence, thus the antibody might also recognize NSP5 from different plant species
    Additional information (application): This antibody will also bind to recombinant KrP (range 10 ng - 1 g)
  • Background
  • Background:
    Kelch repeat-containing protein (KrP)/Galactose oxidase is a protein associated with iron acquisition and metal homeostasis which is a marker for iron defficiency due to the fact that the transcript levels are stably regulated under iron defficiency conditions. Protein can be a target of of bHLH39, bHLH100 and bHLH101 transcription factors. The gene localized at locus At3g07720 is a supposed ancestor of NSP (nitrile-specifier proteins), NSP1, NSP2, and NSP5, which unlike its descendants does not show nitrile-specifier activity.

    Alternative names: NSL1, NSP-like1, nitrile-specifier protein-like 1
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items