KLH | Keyhole Limpet Hemocyanin (monoclonal, clone 6D12)

Product no: AS22 4757
AS22 4757 | Clonality: Monoclonal  | Host: Mouse | Reactivity: KLH | Keyhole Limpet Hemocyanin 
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Delivery:  3-6 business days
  • Product Info
  • Sub class: IgG1 / Kappa
    Immunogen: Hemocyanin from Megathura crenulata.
    Host: Mouse
    Clonality: Monoclonal
    Purity: Purified on Protein A to a total immunoglobulin fraction, in Tris 0,1M, glycine 0,1M and 2 % sucrose.
    Format: Lyophilized
    Quantity: 100 µg
    Reconstitution: Reconstitute in distilled water.
    Storage: Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1:10 000 - 1:100 000 (ELISA), 1: 500 - 1: 5000 (WB)
    Expected | apparent MW: ca, 400 kDa/subunit
  • Reactivity
  • Confirmed reactivity: Reacts with Keyhole Limpet 8-9 kDa Hemocyanin protein.
  • Additional Information
  • Additional information (application): Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.
  • Background
  • Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein  has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells,  monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
  • Protocols
  • Antibody protocols
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