LexA | SOS function regulatory protein

Product no: AS12 2614

AS12 2614  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:Anabaena sp. strain PCC 7120, Synechocystis sp. strain PCC6803

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  • Product Info
  • Immunogen:

    LexA from Anabaena sp. strain PCC 7120 with added His-tag, has been overexpressed in E.coli and purified

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 16 000 (WB)
    Expected | apparent MW:

    26 kDa (for a monomer/subunit)

  • Reactivity
  • Confirmed reactivity: Synechocystis sp. PCC 6803 (gene: sll1626, protein: Sll1626)
    Predicted reactivity: Cyanobacteria
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Antibody can be used as a marker of cytoplasm but also, most likely, a marker for DNA localisation
  • Background
  • Background:

    LexA (EC 3.4.21.88) is a DNA binding protein, is associated with SOS response of cyanobacteria as well as other regulatory networs. Has been shown to directly regulate genes involved in carbon assimilation or controlled by carbon availability. In Synechocystis PCC 6803 LexA is not localised to the thylakoid membranes but to the inner region of the cytoplasm 

     

  • Product Citations
  • Selected references: Oliveira and Lindblad (2011). Novel Insights into the Regulation of LexA in the Cyanobacterium Synechocystis sp. Strain PCC 6803. J. of Bacteriology, Aug. 2011: 3804-3814.
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