LexA | SOS function regulatory protein

AS12 2614 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Anabaena sp. strain PCC 7120, Synechocystis sp. strain PCC6803

Product Information

Immunogen

LexA from Anabaena sp. strain PCC 7120 with added His-tag, has been overexpressed in E.coli and purified

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 16 000 (WB)

Expected | apparent MW

26 kDa (for a monomer/subunit)

Reactivity

Confirmed reactivity Synechocystis sp. PCC 6803 (gene: sll1626, protein: Sll1626)

Predicted reactivity Cyanobacteria
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Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody can be used as a marker of cytoplasm but also, most likely, a marker for DNA localisation

Background

LexA (EC 3.4.21.88) is a DNA binding protein, is associated with SOS response of cyanobacteria as well as other regulatory networs. Has been shown to directly regulate genes involved in carbon assimilation or controlled by carbon availability. In Synechocystis PCC 6803 LexA is not localised to the thylakoid membranes but to the inner region of the cytoplasm

Product citations

Selected references Oliveira and Lindblad (2011). Novel Insights into the Regulation of LexA in the Cyanobacterium Synechocystis sp. Strain PCC 6803. J. of Bacteriology, Aug. 2011: 3804-3814.