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AGO set
Tag Antibody Set 

Lhca5 | PSI type V chlorophyll a/b-binding protein

AS05 082 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants including A.thaliana, H.vulgare, S.oleracea

Lhca5 | PSI type V chlorophyll a/b-binding protein in the group Antibodies for Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS05 082)

PRODUCT INFORMATION IN PDF

Qty: 
332 €

Datasheet Product citations Protocols Add review

 

Product Information

Immunogen

BSA-conjugated synthetic peptide derived from the Lhca5 protein of Arabidopsis thaliana (At1g45474). This sequence is highly conserved in Lhca5 proteins of angiosperms (monocots and dicots) and gymnosperms. UniProt: Q9C639, TAIR: At1g45474

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000-1 : 3000 (WB)
Expected | apparent MW

27.8 | 23 for Arabidopsis thaliana

Reactivity

Confirmed reactivity Arabidopsis thaliana, Bryopsis corticulans, Hordeum vulgare, Spinacia oleracea
Predicted reactivity Dicots
Not reactive in

Chlamydomonas reinhardtii

Application examples

Additional information

Additional information

More information on the Lhca5 localization within the LHCI antenna can be found in Lucinski et al. (2006) Lhca5 interaction with plant photosystem I. FEBS Letters 580:6485-6488

Protein is processed into mature form (Jansson 1999).

Urea is not recommended to use in extraction buffer and in a gel, when working with this antibody.

Related products

Related products

AS01 005 Anti-Lhca1 | PSI type I chlorophyll a/b-binding protein, rabbit antibodies
AS01 006 Anti-Lhca2 | PSI type II chloropyll a/b-binding protein, rabbit antibodies
AS01 007 Anti-Lhca3 | PSI type III chlorophyll a/b-binding protein, rabbit anitbodies
AS01 008
Anti-Lhca4 | PSI type IV chlorophyll a/b-binding protein, rabbit antibodies
LHC  available antibodies against pigment-binding proteins

Plant protein extraction buffer

Secondary antibodies

Background

Background

Lhca5 is a low abundant chlorophyll a/b-binding protein that has been found to associate with Photoystem I in sub-stoechiometric amounts. Unlike the four main Lhca-types (Lhca1-4) Lhca5 seems to be able to associate with PSI or the LHCI at varying positions. The regulation pattern for the expressino of the Lhca5 gene differs from the standard Lhc pattern as expression seems to be positively regulated under light and cold stress conditions.

Product citations

Selected references Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.
Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.

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