Lhcb6 | CP24 chlorphyll a/b-binding protein of plant PSII

AS01 010  |  Clonality: Polyclonal  | Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Brassica napus, Hordeum vulgare, Triticale

Lhcb6 | CP24 chlorphyll a/b-binding protein of plant PSII in the group Antibodies Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS01 010)
Lhcb6 | CP24 chlorphyll a/b-binding protein of plant PSII


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Product Information


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Lhcb6, UniProt: Q9LMQ2, TAIR:At1g15820. This sequence is highly conserved in angiosperms (monocots and dicots) and gymnosperms.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000-1 : 5000 (WB)
Expected | apparent MW

27.5 | 24 kDa for Arabidopsis thaliana


Confirmed reactivity Arabidopsis thaliana, Brassica napus, Hordeum vulgare, Triticum aestivum, Triticale
Predicted reactivity

Dictos, Gymnosperms, Physcomitrium patens, Pisum sativum, Selaginella martensii, Spinacia oleracea, Zea may 

, Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-Lhcb6 antibodies

5 µg of total protein from Arabidopsis thaliana extracted with Agrisera Protein Extraction Buffer PEB (AS08 300) and denatured in PEB at 70°C for 5 min. were separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer (blotted 15h to PVDF using tank-transfer - 30V). Blots were blocked with TBST with 4 % BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS-T with 2% BSA. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1: 50 000 in for 1h at RT with agitation in TBS-T with 2% BSA. The blot was washed as above and developed with chemiluminescence detection reagent for 5 minutes. Exposure time was 25 seconds.

Courtesy of Dr. Robert Luciński, Department of Biology, UAM, Poznań, Poland

Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 32963291

Journal: Sci Rep

Figure Number: 3D

Published Date: 2020-09-22

First Author: Wang, L., Leister, D., et al.

Impact Factor: 4.13

Open Publication

Photomorphogenesis is not significantly affected in ros1, nrpd1, nrpe1, rdr2 or ago4 mutant seedlings. (a) Phenotypes (upper panel) and the corresponding maximum quantum yields of PSII (Fv/Fm) (lower panel) of 4-day-old etiolated seedlings. Fv/Fm was measured with an imaging Chl fluorometer (Imaging PAM). Scale bar?=?1 cm. (b) Phenotypes (upper panel) and corresponding Fv/Fm values (lower panel) of 3-day-old etiolated seedlings which had been exposed to continuous light for 24 h. (c) Immunoblot analysis of the PSII core proteins (D1 and D2), Lhcb1 and FLU during greening of etiolated seedlings. WT seedlings were grown for 3 days in the dark and exposed to light for between 0 and 48 h, as indicated. Extracted total proteins were normalized with respect to fresh weight and fractionated by SDS-PAGE. Blots were then probed with antibodies raised against the individual proteins. Total proteins from 5-day-old WT seedlings grown under continuous light (LL) and LD conditions (LD) were used as positive controls. The total protein accumulation of each sample was visualized by staining the gel with Coomassie Blue R250 (C.B.). The figure was assembled from different blots (delineated by a black rectangle) and full-length blots are presented in Supplementary Fig. S7. (d) Immunoblot analysis of representative photosynthesis proteins of 3-day-old etiolated mutant seedlings which had been exposed to continuous light for 24 h. Immunoblot analysis was performed as in (c). The figure was assembled from different blots (delineated by a black rectangle) and full-length blots are presented in Supplementary Fig. S8. (e) Real-time PCR analyses of 3-day-old etiolated WT (Col-0) and mutant seedlings that had been exposed to continuous light for 24 h. Real-time PCR was performed with primers specific for the nuclear genes LHCB1.2, LHCB2.1, LHCB6, LHCA5, PSBP-1 and PSBTn, and the plastid genes psaA and atpB. Note that the primers for LHCB2.1 also amplify LHCB2.2 mRNA. Expression values are reported relative to the corresponding transcript levels in the WT and were normalized with respect to the expression level of ACTIN2. Data are shown as mean values?ą?SD from three different plant pools.

Additional information

Additional information This product can be sold containing ProClin if requested
Protein is processed into mature form (Jansson 1999).

This antibody is a re-make of former Lhcb6 antibody from Agrisera and is made to the same peptide. 

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Plant protein extraction buffer



Lhcb6 is one of the 3 highly conserved minor chlorophyll a/b-binding proteins exclusively associated with Photosystem II in plants and algae. Together with Lhcb4 and Lhcb5, it regulates the energy flow from the outer antenna to the reaction center through the action of the xanthophyll cycle.

Product citations

Selected references Wójtowicz et al. (2020). Compensation Mechanism of the Photosynthetic Apparatus in Arabidopsis thaliana ch1 Mutants. Int J Mol Sci. 2020 Dec 28;22(1):221. doi: 10.3390/ijms22010221. PMID: 33379339; PMCID: PMC7794896.
Chen et al. (2019). Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat. Int J Mol Sci. 2019 Dec 31;21(1). pii: E268. doi: 10.3390/ijms21010268.
Rogowski et al. (2019). Photosynthesis and organization of maize mesophyll and bundle sheath thylakoids of plants grown in various light intensities. Environmental and Experimental Botany Volume 162, June 2019, Pages 72-86.
Mao et al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.
Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.
Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.
Wang et al. (2018). iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed. Molecular Breeding January 2018, 38:2.
Tyutereva et al. (2017). Stomata control is changed in a chlorophyll b-free barley mutant. Functional Plant Biology,
Chen et al. (2017). Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains. J Plant Growth Regul.

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