Anti-Lhcx (P. tricornutum)

Product no: AS19 4367

AS19 4367 | Clonality:Polyclonal | Host: Rabbit | Reactivity: Pheodactyllum tricornutum

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from C-terminal sequence of Lhcx protein from Phaeodactylum tricornutum. 14 (Lhcx1, Lhcx3), 13 (Lhcx2), and 12 (Lhcx4) out of 15 amino acids long peptide used to elicit this antibody is contained specifically in the C-terminus of the respective Lhcx proteins in P. tricornutum
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 10 000 (WB)
    Expected | apparent MW: Lhcx1: 21.95 | 16 kDa, Lhcx2: 24.73 | 22 kDa, Lhcx3: 22.24 | 17-18 kDa, Lhc4: 22.86 | 17-18 kDa. However, based on other marker and gel systems, Lhcx1 and Lhcx3/4 may also appear at 18 and 19-20 kDa.
  • Reactivity
  • Confirmed reactivity: Phaeodactylum tricornutum
    Predicted reactivity: Cyclotella cryptica, Cytocella meneghiniana, Durinskia baltica, Fragilariopsis cyclindrus, Thalassioria pseudonana, Pseudo-nitzschia multistriata, Vitrella brassicaformis CCMP3155

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  • Additional Information
  • Additional information: This item can be sold containing ProClin
  • Background
  • Background: P. tricornutum contains only four Lhcx proteins, which are are structurally similar, but are differentially expressed under varying environmental conditions.
  • Product Citations
  • Selected references: Buck et al. (2021) Identification of sequence motifs in Lhcx proteins that confer qE-based photoprotection in the diatom Phaeodactylum tricornutum. Plant J. 2021 Dec;108(6):1721-1734. doi: 10.1111/tpj.15539. Epub 2021 Nov 3. PMID: 34651379.
    Buck et al. (2019). Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum. Nat Commun. 2019 Sep 13;10(1):4167. doi: 10.1038/s41467-019-12043-6.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items