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Anti-LSD1 | Lesion simulating disease 1 (chicken antibody)
Product no: AS12 2104
AS12 2104 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabidopsis thaliana
244
€
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Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana LSD1 sequence, UniProt: P94077, TAIR: AT4G20380.
Host: Chicken Clonality: Polyclonal Purity: Immunogen affinity purified IgY in PBS pH 7.4. Contains 0.02% sodium azide. Format: Liquid Quantity: 50 µg Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 19.5 kD - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Brassica olereacea, Pisum sativum
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
50 g of total protein from Arabidopsis thaliana wilde type, lsd1 mutant and transgenic plants overexpressin LSD1-GFP protein under 35S promotor were extracted with following extraction buffer: 0.0625 M Tris-HCl pH 6.8, 1 % SDS, 10 % glycerol, 0.01% beta-mercaptoethanol) were separated in 10 % SDS-PAGE and blotted for 16 hours to PVDF at 4°C using tank transfer. Blots were blocked in PBS-T with 4.5 % non-fat milk for 4 hours at room temperature with agitation. Blot was incubated in the primary antibody at a dilution of 1. 500 for 20 hours at 4°C with agitation. The antibody solution was decanted and the blot was washed 5x for 10 minutes in PBS-T at RT with agitation. Blot was incubated in secondary antibody, anti-chicken IgY, HRP conjugated diluted to 1: 7500 in PBS for 1 hour at RT with agitation. The blot was washed as above and developed for 5 minutes with ECL according to the manufacture's instruction. Exposure time was 1 minute.
Courtesy of Dr. Keun Pyo Lee, SIBS, China - Additional Information
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Additional information (application): This antibody has so far confirmed reactivity on recombinant LSD1 - Background
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Background: LSD1 (Lesion simulating disease 1) monitors a superoxide-dependent signal and negatively regulates a plant cell death pathway. contains zinc-finger motifs. LSD1 negatively regulates a basal defense pathway that can act upstream or independently of both NIM1/NPR1 function and SA accumulation. Alternative names: CHILLING SENSITIVE 4, CHS4. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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