LSD1 | Lesion simulating disease 1 (chicken antibody)

AS12 2104 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana LSD1 sequence, UniProt: P94077, TAIR: AT4G20380.

Host Chicken

Clonality Polyclonal

Purity Affinity purfied IgY in PBS pH 7,4 with 0,02 % sodium azide

Format Liquid

Quantity 50 µg

Storage Store at 4°C; make aliquots to avoid working with a stock, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 19,5 kD

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Brassica olereacea, Pisum sativum
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

50 g of total protein from Arabidopsis thaliana wilde type, lsd1 mutant and transgenic plants overexpressin LSD1-GFP protein under 35S promotor were extracted with following extraction buffer: 0.0625 M Tris-HCl pH 6.8, 1 % SDS, 10 % glycerol, 0.01% beta-mercaptoethanol) were separated in 10 % SDS-PAGE and blotted for 16 hours to PVDF at 4°C using tank transfer. Blots were blocked in PBS-T with 4.5 % non-fat milk for 4 hours at room temperature with agitation. Blot was incubated in the primary antibody at a dilution of 1. 500 for 20 hours at 4°C with agitation. The antibody solution was decanted and the blot was washed 5x for 10 minutes in PBS-T at RT with agitation. Blot was incubated in secondary antibody, anti-chicken IgY, HRP conjugated diluted to 1: 7500 in PBS for 1 hour at RT with agitation. The blot was washed as above and developed for 5 minutes with ECL according to the manufacture's instruction. Exposure time was 1 minute.

Courtesy of Dr. Keun Pyo Lee, SIBS, China

Additional information

This antibody has so far confirmed reactivity on recombinant LSD1

Related products

Plant protein extraction buffer

Secondary antibodies

Background

Background LSD1 (Lesion simulating disease 1) monitors a superoxide-dependent signal and negatively regulates a plant cell death pathway. contains zinc-finger motifs. LSD1 negatively regulates a basal defense pathway that can act upstream or independently of both NIM1/NPR1 function and SA accumulation. Alternative names: CHILLING SENSITIVE 4, CHS4.