MDH2 | Malate dehydrogenase 2 (mitochondrial)

AS15 3064 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Solanum lycopersicum, Zea mays

MDH2 | Malate dehydrogenase 2 (mitochondrial)

Product Information

Immunogen

Recombinant MDH2 of Zea mays, UniProt: B4FZU8

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

35 | 35 kDa

Reactivity

Confirmed reactivity Solanum lycopersicum, Zea mays

Predicted reactivity Arabidopsis thaliana, Brachypodium distachyon, Citrus sinensis, Coffea canephora, Cucumis sativus, Glycine max, Gossypium raimondii , Hordeum vulgare, Jatropha curcas, Leersia perrieri, Morus notabilis, Oryza sativa, Phaseolus vulgaris , Populus trichocarpa, Prunus persica, Ricinus communis, Setaria italica, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Zostera marina, Vitis vinifera
Species of your interest not listed? Contact us

Application examples

Application examples Application example

Zea mays crude extract (1), Zea mays mitochondria enriched fraction (2), immunoprecipitated sample (3), recombinant MHD4, expressed inE.coli (4). Total maize leaf proteins were extracted from 600 mg frozen leaf material with 1:4 ratio using 2.4 ml of extraction buffer (50 mM Trsi-HCl pH 8.0, 10 mM Ascorbate, 2 mM EDTA and 330 mM Mannitol, 1 x protease Inhibitor cocktail (Sigma Aldrich P8849-5ML). The homogenate was produced by mortar and pestle and centrifuged at 4000 x g for 10 min. at 4°C. The SDS-sample buffer (lane 1 in SDS-PAGE) is related to extraction using directly mortar and pestle and 2.4 ml of SDS-sampe buffer (0.1 M Tris-HCl pH 8.0, 100 mM DTT, 2 mM EDTA, 30% glycerol, 0.05 % bromophenolblue). The supernatant of 4000 x g was further centrifuged at 20,000 x g for 3 min in order to remove the chloroplasts. SDS-PAGE was performed using NuPAGE 4-12% (Invitrogen) at 120 V for 1.2 hours and blotted for 1h to Nitrocellulose membrane (BIOTrace NT, PALL, Life Sciences) using semi-dry transfer apparatus (Hoeffer) at 45 Ampere x 2 (gels) and variable voltage. Blots were blocked with Genscript 5M Quick Blocking solution (One step western blot kit, mix of solution A, casein hydrolysate and B, TTBS 1:1) for 15 minutes at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 overnight at RT with agitation (sealed in a bag). The antibody solution was decanted and the blot was rinsed briefly twice with TTBS, then washed 3 times for 10 min in TTBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:5000 in TTBS for 45 minutes at RT with agitation. The blot was washed as above and developed for 5 min with NBT/BCIP Ready-to-Use Tablets. Color development was stopped by decanting the solution and replacing with distilled water containing 10 mM EDTA. The membranes were dried on paper and photographed. Samples (boiled in boiling water for 10 minutes): 1) crude leaf extract by SDS-sample buffer (centrifuged 4000 x g for 10 min. at 4°C); Loaded 10µl 2) Leaf, mitochondria enriched supernatant 20,000 x g for 3 min. at 4°C; Loaded 10µl 3) Immunoprecipitation (IP) samples (tot. vol= 100µl); Loaded 8µl 4) Recombinant protein 1 µg/µl; loaded 1µl;

Courtesy of Dr. Giuseppe Dionisio, Aarhus University, Danmark

Additional information

Immunoprecipitation was performed by using Dynabeads ® Protein A: briefly 100 µl suspension was washed with 200 µl TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7,6 and 165 mM NaCl with 0,1% Tween 80) using the magnetic stands for concentrating the magnetic beads, After wash the beads were preincubated with 20 µl primary antibodies in 180 µl TTBS at room temperature for 30 minutes (minimum 15 minues), A first wash was followed afterwards with 200 µl TTBS and hence a real incubation with 200 µl plant extract (supernatant 20,000 x g for 3 min,), 200µl of TTBS and further 50 µl YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane, This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension, Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 µl TTBS were performed prior mixing with 100 µl SDS-Sample buffer

Background

Malate dehydrogenase (EC=1.1.1.37) is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate. This reaction is part of many metabolic pathways, including the citric acid cycle. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. The protein is highly expressed in: young panicles and immature seeds, while its levels in roots and leaves are low. Alternative name: PP37.

Product citations

Selected references Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.

All references:	Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.
calculated \| apparent molecular mass [kDa]:	35 \| 35 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant MDH2 of Zea mays, UniProt: B4FZU8
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunoprecipitation (IP), Western blot (WB)
background:	Malate dehydrogenase (EC=1.1.1.37) is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate. This reaction is part of many metabolic pathways, including the citric acid cycle. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. The protein is highly expressed in: young panicles and immature seeds, while its levels in roots and leaves are low. Alternative name: PP37.
additional information (application):	Immunoprecipitation was performed by using Dynabeads ® Protein A: briefly 100 µl suspension was washed with 200 µl TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7,6 and 165 mM NaCl with 0,1% Tween 80) using the magnetic stands for concentrating the magnetic beads, After wash the beads were preincubated with 20 µl primary antibodies in 180 µl TTBS at room temperature for 30 minutes (minimum 15 minues), A first wash was followed afterwards with 200 µl TTBS and hence a real incubation with 200 µl plant extract (supernatant 20,000 x g for 3 min,), 200µl of TTBS and further 50 µl YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane, This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension, Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 µl TTBS were performed prior mixing with 100 µl SDS-Sample buffer
Confirmed reactivity:	Solanum lycopersicum, Zea mays
predicted reactivity:	Arabidopsis thaliana, Brachypodium distachyon, Citrus sinensis, Coffea canephora, Cucumis sativus, Glycine max, Gossypium raimondii , Hordeum vulgare, Jatropha curcas, Leersia perrieri, Morus notabilis, Oryza sativa, Phaseolus vulgaris , Populus trichocarpa, Prunus persica, Ricinus communis, Setaria italica, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Zostera marina, Vitis vinifera Species of your interest not listed? Contact us
Picture (footer):	Application example Zea mays crude extract (1), Zea mays mitochondria enriched fraction (2), immunoprecipitated sample (3), recombinant MHD4, expressed inE.coli (4). Total maize leaf proteins were extracted from 600 mg frozen leaf material with 1:4 ratio using 2.4 ml of extraction buffer (50 mM Trsi-HCl pH 8.0, 10 mM Ascorbate, 2 mM EDTA and 330 mM Mannitol, 1 x protease Inhibitor cocktail (Sigma Aldrich P8849-5ML). The homogenate was produced by mortar and pestle and centrifuged at 4000 x g for 10 min. at 4°C. The SDS-sample buffer (lane 1 in SDS-PAGE) is related to extraction using directly mortar and pestle and 2.4 ml of SDS-sampe buffer (0.1 M Tris-HCl pH 8.0, 100 mM DTT, 2 mM EDTA, 30% glycerol, 0.05 % bromophenolblue). The supernatant of 4000 x g was further centrifuged at 20,000 x g for 3 min in order to remove the chloroplasts. SDS-PAGE was performed using NuPAGE 4-12% (Invitrogen) at 120 V for 1.2 hours and blotted for 1h to Nitrocellulose membrane (BIOTrace NT, PALL, Life Sciences) using semi-dry transfer apparatus (Hoeffer) at 45 Ampere x 2 (gels) and variable voltage. Blots were blocked with Genscript 5M Quick Blocking solution (One step western blot kit, mix of solution A, casein hydrolysate and B, TTBS 1:1) for 15 minutes at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 overnight at RT with agitation (sealed in a bag). The antibody solution was decanted and the blot was rinsed briefly twice with TTBS, then washed 3 times for 10 min in TTBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:5000 in TTBS for 45 minutes at RT with agitation. The blot was washed as above and developed for 5 min with NBT/BCIP Ready-to-Use Tablets. Color development was stopped by decanting the solution and replacing with distilled water containing 10 mM EDTA. The membranes were dried on paper and photographed. Samples (boiled in boiling water for 10 minutes): 1) crude leaf extract by SDS-sample buffer (centrifuged 4000 x g for 10 min. at 4°C); Loaded 10µl 2) Leaf, mitochondria enriched supernatant 20,000 x g for 3 min. at 4°C; Loaded 10µl 3) Immunoprecipitation (IP) samples (tot. vol= 100µl); Loaded 8µl 4) Recombinant protein 1 µg/µl; loaded 1µl; Courtesy of Dr. Giuseppe Dionisio, Aarhus University, Danmark