MME4 | Malic enzyme

Product no: AS12 2617

AS12 2617  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

381
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii MME4 protein seqeunce A8IUZ4. The peptide is also conserved in MME3 A8IUW2

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 200 g
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    56 kDa

  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii
    Predicted reactivity: Chlamydomonas reinhardtii
    Not reactive in:

    Chromera velia

  • Application Examples
  • application example

    western blot using anti-MME4 antibody

    25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions and dark anoxia were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation in a secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T.  The blot was washed and developed with alkaline phosphatase color development reagent according to the manufacturer's instructions. 

    Courtesy of Dr. Alexandra Dubini



  • Background
  • Background:

    MME4 (malic enzyme) belongs to the malic enzyme family.

  • Product Citations
  • Selected references: Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21.
  • Protocols


  • Reviews:
  • To leave a review, you should have previously purchased this product and to your customer profile.

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
Add to cart
Info
img missing
AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S

This product can be purchased in 3 different volumes:

AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer

AS16 ECL-SN, 100 ml

AS16 ECL-SN-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 26 €

AS16 3966 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

302 €
Add to cart
Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS12 2618  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

 

381 €
Add to cart
Info
Close