1
MME4 | Malic enzyme
AS12 2617 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Product Info
-
Immunogen: KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii MME4 protein seqeunce A8IUZ4. The peptide is also conserved in MME3 A8IUW2
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 200 µg Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 56 kDa
- Reactivity
-
Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Chlamydomonas reinhardtii Not reactive in: Chromera velia
- Application Examples
-
application example
25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions and dark anoxia were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation in a secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T. The blot was washed and developed with alkaline phosphatase color development reagent according to the manufacturer's instructions.
Courtesy of Dr. Alexandra Dubini
- Background
-
Background: MME4 (malic enzyme) belongs to the malic enzyme family.
- Product Citations
-
Selected references: Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21. - Protocols
-
- Reviews:
-
To leave a review, you should have previously purchased this product and log in to your customer profile.
This product doesn't have any reviews.
Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 % until end of 2023. Use discount code: Conj10
Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S
This product can be purchased in 3 different volumes:
AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer
AS16 ECL-SN, 100 ml
AS16 ECL-SN-1L, 1L
Choose the appropriate volume in the drop down menu to the right
AS16 3966 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS12 2618 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii