MME4 | Malic enzyme

AS12 2617 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

Product Information

Immunogen

KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii MME4 protein seqeunce A8IUZ4. The peptide is also conserved in MME3 A8IUW2

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

56 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Chlamydomonas reinhardtii

Not reactive in

Chromera velia

Application examples

application example

25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions and dark anoxia were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation in a secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T. The blot was washed and developed with alkaline phosphatase color development reagent according to the manufacturer's instructions.

Courtesy of Dr. Alexandra Dubini

Additional information

Background

MME4 (malic enzyme) belongs to the malic enzyme family.

Product citations

Selected references Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21.