MME4 | Malic enzyme
AS12 2617 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

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Product Information
KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii MME4 protein seqeunce A8IUZ4. The peptide is also conserved in MME3 A8IUW2
56 kDa
Reactivity
Chromera velia
Application examples
application example
25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions and dark anoxia were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation in a secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T. The blot was washed and developed with alkaline phosphatase color development reagent according to the manufacturer's instructions.
Courtesy of Dr. Alexandra Dubini
Additional information
Background
MME4 (malic enzyme) belongs to the malic enzyme family.
Product citations
Related products: MME4 | Malic enzyme
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