FMR | Fumarate reductase

Product no: AS12 2618

AS12 2618  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

 

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  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii FMR protein seqeunce, UniProt: A8IQY2
    protein ID 145357.
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 200 g
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    40 kDa

  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii
    Predicted reactivity: Gonium pectorale, Leishmania mexicana, Naegleria gruberi (Amoeba), Nannochloropsis gaditana
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example


    western blot using anti-FMR antibody

    25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions (A), dark anoxia (B) were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation ina secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T.  The blot was washed   and developed with alkaline phosphatase color development reagent according to the manufacturer's instructions.

    Courtesy of Dr. Alexandra Dubini

  • Background
  • Background:

    FMR (fumarate reductase) is an enzyme that catalyze the final steps of succinate synthesis.

  • Product Citations
  • Selected references: Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21.
  • Protocols


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AS12 2618  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

 

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