FMR | Fumarate reductase
AS12 2618 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
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protein ID 145357.
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25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions (A), dark anoxia (B) were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation ina secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T. The blot was washed and developed with alkaline phosphatase color development reagent according to the manufacturer's instructions.
Courtesy of Dr. Alexandra Dubini
FMR (fumarate reductase) is an enzyme that catalyze the final steps of succinate synthesis.