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FMR | Fumarate reductase

AS12 2618  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

 

FMR | Fumarate reductase in the group Antibodies for Plant/Algal  / Chlamydomonas reinhardtii at Agrisera AB (Antibodies for research) (AS12 2618)

DATA SHEET IN PDF

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360
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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii FMR protein seqeunce, UniProt: A8IQY2
protein ID 145357.
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

40 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Gonium pectorale, Leishmania mexicana, Naegleria gruberi (Amoeba), Nannochloropsis gaditana
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example


western blot using anti-FMR antibody

25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions (A), dark anoxia (B) were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation ina secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T.  The blot was washed   and developed with alkaline phosphatase color development reagent according to the manufacturer's instructions.

Courtesy of Dr. Alexandra Dubini

Additional information

Related products

Related products

AS12 1617 | Anti-MME4 | malic enzyme, rabbit antibodies

Algal protein extraction buffer

Secondary antibodies

Background

Background

FMR (fumarate reductase) is an enzyme that catalyze the final steps of succinate synthesis.

Product citations

Selected references Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21.

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