FMR | Fumarate reductase

Name: FMR | Fumarate reductase
SKU: AS12 2618
Price: 381 EUR
Availability: InStock

AS12 2618 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

FMR | Fumarate reductase in the group Antibodies Plant/Algal / Chlamydomonas reinhardtii at Agrisera AB (Antibodies for research) (AS12 2618)

Product Information

Immunogen KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii FMR protein seqeunce, UniProt: A8IQY2
protein ID 145357.

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

40 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Gonium pectorale, Leishmania mexicana, Naegleria gruberi (Amoeba), Nannochloropsis gaditana
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example

western blot using anti-FMR antibody

25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions (A), dark anoxia (B) were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation ina secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T. The blot was washed and developed with alkaline phosphatase color development reagent according to the manufacturer's instructions.

Courtesy of Dr. Alexandra Dubini

Additional information

Background

FMR (fumarate reductase) is an enzyme that catalyze the final steps of succinate synthesis.

Product citations

Selected references Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21.

immunogen:	KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii FMR protein seqeunce, UniProt: A8IQY2 protein ID 145357.
Reconstitution:	For reconstitution add 200 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	200 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	40 kDa
Confirmed reactivity:	Chlamydomonas reinhardtii
predicted reactivity:	Gonium pectorale, Leishmania mexicana, Naegleria gruberi (Amoeba), Nannochloropsis gaditana Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example 25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions (A), dark anoxia (B) were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation ina secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T. The blot was washed and developed with alkaline phosphatase color development reagent according to the manufacturer's instructions. Courtesy of Dr. Alexandra Dubini
background:	FMR (fumarate reductase) is an enzyme that catalyze the final steps of succinate synthesis.
All references:	Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21.