NBR1 | Autophagy substrate NBR1
AS14 2805 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Physcomitrella patens

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75 | 100 kDa
Reactivity
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Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 29079776
Journal: Nat Commun
Figure Number: 5A
Published Date: 2017-10-27
First Author: Dong, Y., Silbermann, M., et al.
Impact Factor: 13.783
Open PublicationAutophagy is specifically induced by limited S-precursor supply for cysteine biosynthesis. a Autophagy induction in the shoot of serat tko and sir1-1 was determined by immunological detection of the canonical autophagy marker ATG8a and NBR1 with specific antisera. Lipidation of ATG8a (ATG8a-PE) (apparent size: 15–20?kDa) is essential for autophagosome formation and indicated by a significant shift during electrophoresis. NBR1 is a cargo receptor for selective autophagy and consequently degraded in autophagic bodies (D-NBR1) (apparent size: 50–75?kDa). b Level of ATG8a-PE and D-NBR1 shown in a were quantified (n?=?3, mean?ą?s.e.m., one-way ANOVA, *p?0.05). c Autophagy induction in the root of serat tko and sir1-1 was detected by MDC staining. White arrows marked the visible autophagic bodies. Scale bar, 25?ĩm. d Autophagy induction in the shoot of WT under sulfur deficiency was determined by ATG8a-PE level (n?=?3, mean?ą?s.e.m., t-test, *p?0.05)
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Product citations
Calero-MuÃ?Âąoz et al. (2019). Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 Sep;42(9):2696-2714. doi: 10.1111/pce.13597.
Jia et al. (2019). Noncanonical ATG8-ABS3 interaction controls senescence in plants. Nat Plants. 2019 Feb;5(2):212-224. doi: 10.1038/s41477-018-0348-x.
Hackenberg et al. (2013). Catalase and NO CATALASE ACTIVITY1 promote autophagy-dependent cell death in Arabidopsis. Plant Cell. 2013 Nov;25(11):4616-26. doi: 10.1105/tpc.113.117192. Epub 2013 Nov 27.
Minina et al. (2013). Autophagy mediates caloric restriction-induced lifespan extension in Arabidopsis. Aging Cell. 2013 Apr;12(2):327-9. doi: 10.1111/acel.12048. Epub 2013 Feb 28. (method description in supplemental materials)
Katsiarimpa et al. (2013). The Deubiquitinating Enzyme AMSH1 and the ESCRT-III Subunit VPS2.1 Are Required for Autophagic Degradation in Arabidopsis.
Svenning et al. (2011). Plant NBR1 is a selective autophagy substrate and a functional hybrid of the mammalian autophagic adapters NBR1 and p62/SQSTM1. Autophagy. 2011 Sep;7(9):993-1010. Epub 2011 Sep 1. (original reference)
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