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3-nitroY | Nitrotyrosine (25 ĩg)

AS10 706-25  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity:nitrotyrosine

3-nitroY | Nitrotyrosine (25 ĩg) in the group Antibodies Plant/Algal / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS10 706-25)
3-nitroY | Nitrotyrosine (25 ĩg)



DATA SHEET IN PDF

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223 €
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl
acetamido) propionic acid
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG2A
Purity Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
Format Liquid
Quantity 25 ĩg
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Flowcyt (Flow cytometry), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator

Reactivity

Confirmed reactivity Dog, Human, Mouse, Rat
Predicted reactivity Higher plants
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

IHC using anti-nitrotyrosine antibodies

IHC using anti-Nitrotyrosine mouse monoclonal antibodies, Clone 39B6. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Anti-Nitrotyrosine mouse monoclonal antibody Dilution: 1:1000000 for 12 h/4°C. Secondary Antibody: goat anti-Mouse biotin conjugated at 1:2000 1 h/RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.

Additional information

Additional information 1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol

The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific.

0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot.

Antibody works paraffin-embedded sections. 

Related products

Background

Background

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.

Product citations

Selected references Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:
Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.
Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
additional information: 1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol
additional information (application):

The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific.

0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot.

Antibody works paraffin-embedded sections. 
All references: Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:
Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.
Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
background:

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
Confirmed reactivity: Dog, Human, Mouse, Rat
predicted reactivity: Higher plants
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Clonality: Monoclonal
Format: Liquid
Host: Mouse
immunogen:

BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl
acetamido) propionic acid
Purity: Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
Quantity: 25 ĩg
recommended dilution: 1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class: IgG2A
tested applications: ELISA (ELISA), Flowcyt (Flow cytometry), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Picture (footer): Application example

IHC using anti-nitrotyrosine antibodies

IHC using anti-Nitrotyrosine mouse monoclonal antibodies, Clone 39B6. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Anti-Nitrotyrosine mouse monoclonal antibody Dilution: 1:1000000 for 12 h/4°C. Secondary Antibody: goat anti-Mouse biotin conjugated at 1:2000 1 h/RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.

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