Name:
Phone:
E-mail:
Message


 


3-nitroY | Nitrotyrosine (25 ĩg)

AS10 706-25  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity:nitrotyrosine

3-nitroY | Nitrotyrosine (25 ĩg)  in the group Antibodies for Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS10 706-25)

DATA SHEET IN PDF

Qty: 
210
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl
acetamido) propionic acid

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG2A
Purity Protein G purified, in PBS with 50 % glycerol, 0.09% sodium azide.
Format Liquid
Quantity 25 µg
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Flowcyt (Flow cytometry), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 100 (IHC), 1: 1400 (WB). The exact and optimal working dilution should be determined by the investigator.

Reactivity

Confirmed reactivity Dog, Human, Mouse, Rat
Predicted reactivity Higher plants
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

IHC using anti-nitrotyrosine antibodies

IHC using anti-Nitrotyrosine mouse monoclonal antibodies, Clone 39B6. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Anti-Nitrotyrosine mouse monoclonal antibody Dilution: 1:1000000 for 12 h/4°C. Secondary Antibody: goat anti-Mouse biotin conjugated at 1:2000 1 h/RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.

Additional information

Additional information

1 mg/ml of Protein G purified IgG2A in PBS pH 7.4,  0.09 % sodium azide, 50 % glycerol

The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific.

0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot.

Antibody works paraffin-embedded sections. 

Related products

Related products

AS10 706-100 | anti-nitrotyrosine mouse antibody, 100 µg

Secondary antibodies

Background

Background

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.

Product citations

Selected references Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:
Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.
Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.

Related products: 3-nitroY | Nitrotyrosine (25 ĩg)

AS10 706-100  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: nit...
415 €
AS16 ECL-S | extreme low femtogram detection
- 10ml trial size is restricted to one unit per cust...
From 10 €
AS09 627 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L...
195 €