Anti-3-nitroY | Nitrotyrosine (25 µg)

Product no: AS10 706-25

AS10 706-25  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity:nitrotyrosine

Add to cart
Delivery:  3-6 business days
  • Product Info
  • Sub class: IgG2A

    BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl
    acetamido) propionic acid

    Host: Mouse
    Clonality: Monoclonal
    Purity: Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
    Format: Liquid
    Quantity: 25 µg
    Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Flowcyt (Flow cytometry), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: 1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
  • Reactivity
  • Confirmed reactivity: Dog, Human, Mouse, Rat
    Predicted reactivity: Higher plants
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    IHC using anti-nitrotyrosine antibodies

    IHC using anti-Nitrotyrosine mouse monoclonal antibodies, Clone 39B6. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Anti-Nitrotyrosine mouse monoclonal antibody Dilution: 1:1000000 for 12 h/4°C. Secondary Antibody: goat anti-Mouse biotin conjugated at 1:2000 1 h/RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
  • Additional Information
  • Additional information: 1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol
    Additional information (application):

    The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific.

    0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot.

    Antibody works paraffin-embedded sections. 

  • Background
  • Background:

    Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.

  • Product Citations
  • Selected references: Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:
    Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.
    Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.


AS09 627 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L)

218 €
Add to cart

AS10 706-100  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: nitrotyrosine

464 €
Add to cart
img missing
AS16 ECL-S | extreme low femtogram detection  |  Limited stock

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

Choose the appropriate volume in the drop down menu to the right

From 17 €