NodGS | Nodulin / glutamate-ammonia ligase-like protein (affinity purified)

AS15 3030A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from a C-terminal of Arabidopsis thaliana NodGS, UniProt: Q8W473 , TAIR: AT3G53180

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Liquid with glycerol at 50 %

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunoprecipitation (IP), Immunofluorescence (IF), Western blot (WB)

Recommended dilution 1 : 300-1 :400 (IP), 1 : 3000 (IF), 1 : 2000 (WB)

Expected | apparent MW

94 | 90 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Medicago truncatula, Theobroma cacao
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

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Background

NodGS (Nodulin/glutamate-ammonia ligase-like protein) shares homology with nodulins and self-assembles into oligomers which are present in response to flagellin treatment. It contains a C-terminal domain of a prokaryotic glutamine synthetase type I. Protein is mainly localized to cytoplasm and is present in a oligomeric form of ca. 700 kDa.

Product citations

Selected references Doskocilova et al. (2011). A nodulin/glutamine synthetase-like fusion protein is implicated in the regulation of root morphogenesis and in signalling triggered by flagellin. Planta 234, 459–476. doi: 10.1007/s00425-011-1419-7