NodGS | Nodulin / glutamate-ammonia ligase-like protein (affinity purified)

AS15 3030A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


product information
Background		NodGS (Nodulin/glutamate-ammonia ligase-like protein) shares homology with nodulins and self-assembles into oligomers which are present in response to flagellin treatment. It contains a C-terminal domain of a prokaryotic glutamine synthetase type I. Protein is mainly localized to cytoplasm and is present in a oligomeric form of ca. 700 kDa.
Immunogen		KLH-conjugated synthetic peptide derived from a C-terminal of Arabidopsis thaliana NodGS, UniProt: Q8W473 , TAIR: AT3G53180
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinity purified serum in PBS, pH 7.4
Format		Liquid with glycerol at 50 %
Quantity		50 µg
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Immunoprecipitation (IP), Immunofluorescence (IF), Western blot (WB)
Related products		AS05 068 \| anti-GDH1 \| glutamate dehydrogenase 1, rabbit antibody AS08 296 GLN \| anti-GS2, chloroplastic form of glutamine synthetase, rabbit antibody AS08 295PRE \| GLN1 GLN2 \| GS1 glutamine synthetase, pre-immune serum collection of antibodies to proteins involved in nitrogen metabolism plant and algal protein extraction buffer secondary antibodies
Additional information

application information
Recommended dilution		1 : 300-1 :400 (IP), 1 : 3000 (IF), 1 : 2000 (WB)
Expected \| apparent MW		94 \| 90 kDa
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity		Medicago truncatula, Theobroma cacao
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		Doskocilova et al. (2011). A nodulin/glutamine synthetase-like fusion protein is implicated in the regulation of root morphogenesis and in signalling triggered by flagellin. Planta 234, 459–476. doi: 10.1007/s00425-011-1419-7

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