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NodGS | Nodulin / glutamate-ammonia ligase-like protein (affinity purified)

AS15 3030A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

NodGS | Nodulin / glutamate-ammonia ligase-like protein (affinity purified) in the group Antibodies Plant/Algal / Nitrogen Metabolism at Agrisera AB (Antibodies for research) (AS15 3030A)
NodGS | Nodulin / glutamate-ammonia ligase-like protein (affinity purified)



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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from a C-terminal of Arabidopsis thaliana NodGS, UniProt: Q8W473 , TAIR: AT3G53180
Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4. with glycerol at 50 %.
Format Liquid
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunoprecipitation (IP), Immunofluorescence (IF), Western blot (WB)
Recommended dilution 1 : 300-1 :400 (IP), 1 : 3000 (IF), 1 : 2000 (WB)
Expected | apparent MW

94 | 90 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Medicago truncatula, Theobroma cacao
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Related products

Background

Background

NodGS (Nodulin/glutamate-ammonia ligase-like protein) shares homology with nodulins and self-assembles into oligomers which are present in response to flagellin treatment.  It contains a C-terminal domain of a prokaryotic glutamine synthetase type I. Protein is mainly localized to cytoplasm and is present in a oligomeric form of ca. 700 kDa.

Product citations

Selected references Doskocilova et al. (2011). A nodulin/glutamine synthetase-like fusion protein is implicated in the regulation of root morphogenesis and in signalling triggered by flagellin. Planta 234, 459–476. doi: 10.1007/s00425-011-1419-7
immunogen:

KLH-conjugated synthetic peptide derived from a C-terminal of Arabidopsis thaliana NodGS, UniProt: Q8W473 , TAIR: AT3G53180
Reconstitution: For reconstitution add 50 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified serum in PBS pH 7.4. with glycerol at 50 %.
Format: Liquid
Quantity: 50 ĩg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Immunoprecipitation (IP), Immunofluorescence (IF), Western blot (WB)
recommended dilution: 1 : 300-1 :400 (IP), 1 : 3000 (IF), 1 : 2000 (WB)
calculated | apparent molecular mass [kDa]:

94 | 90 kDa
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Medicago truncatula, Theobroma cacao
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background:

NodGS (Nodulin/glutamate-ammonia ligase-like protein) shares homology with nodulins and self-assembles into oligomers which are present in response to flagellin treatment.  It contains a C-terminal domain of a prokaryotic glutamine synthetase type I. Protein is mainly localized to cytoplasm and is present in a oligomeric form of ca. 700 kDa.
All references: Doskocilova et al. (2011). A nodulin/glutamine synthetase-like fusion protein is implicated in the regulation of root morphogenesis and in signalling triggered by flagellin. Planta 234, 459–476. doi: 10.1007/s00425-011-1419-7

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