NodGS | Nodulin / glutamate-ammonia ligase-like protein

AS15 3030 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

NodGS | Nodulin / glutamate-ammonia ligase-like protein

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from a C-terminal of Arabidopsis thaliana NodGS, UniProt: Q8W473 , TAIR: AT3G53180

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunoprecipitation (IP), Immunofluorescence (IF), Western blot (WB)

Recommended dilution 1 : 300-1 :400 (IP), 1 : 3000 (IF), 1 : 2000 (WB)

Expected | apparent MW

94 | 90 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Medicago truncatula, Theobroma cacao
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-NodGS antibodies

4,5 of total protein (supernatant after 1h at 25.000 xg) from Arabidopsis thaliana suspension culture extracted with 50mM HEPES pH 7,5/75mM NaCl/1mM EGTA/1mM MgCl2/1mM NaF in the presence of protease inhibitors were separated on 8 % SDS-PAGE and blotted 1,5h to a nitrocellulose membrane using tank transfer. Blots were blocked with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500, 1:1000, 1:2.000, and 1:5000 overnight at 4°C without agitation. The antibody solution was decanted and the blot was rinsed, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 5% milk for 30 min at RT with agitation. The blot was washed as above and developed for 1 min with chemiluminescent detection reagent. Exposure time was 15 seconds.

Courtesy of Dr. Jana Chumová, Laboratoř funkční cytologie, Czech Republic

Additional information

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Background

NodGS (Nodulin/glutamate-ammonia ligase-like protein) shares homology with nodulins and self-assembles into oligomers which are present in response to flagellin treatment. It contains a C-terminal domain of a prokaryotic glutamine synthetase type I. Protein is mainly localized to cytoplasm and is present in a oligomeric form of ca. 700 kDa.

Product citations

Selected references Doskocilova et al. (2011). A nodulin/glutamine synthetase-like fusion protein is implicated in the regulation of root morphogenesis and in signalling triggered by flagellin. Planta 234, 459–476. doi: 10.1007/s00425-011-1419-7