IKP

Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)

Product no: AS20 4387

AS20 4387   | Clonality: Recombinant Monoclonal |  Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV

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  • Product Info
  • Immunogen: Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa), UniProt: P0DTC9

    Sub class: Mouse scFv fusion with human IgG1 Fc
    Host:

    Mouse

    Clonality: Monoclonal recombinant (clone 1A6)
    Purity: Affinity chrompatography purified in 10 mM PBS, pH 7.4, 50 % glycerol, 0.03% Proclin 300.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at -20C or -80C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1: 10 000 - 1: 50 000 (ELISA), 1: 500-1: 5000 (WB)
    Expected | apparent MW: 45 kDa
  • Reactivity
  • Confirmed reactivity: Human Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    Western blot using anti-Cov19 Nucleaocapsid protein N

    200 ng of  His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein overexpressed in E. coli was separated on a 10 % SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5 % non-fat milk and following a series of washes with TBS-T and incubated with anti-SARS-CoV-2 nucleocapsid antibodies at a dilution of 1:1000 at RT for 2 h. Following a series of washes with TBS-T the membrane was incubated with a secondary antibody, goat anti-human IgG, Fc fragment at 1: 20 000 for 1h/RT. Following a standard series of washes with TBS-T, reaction was visualised with chemiluminescence following manufacture's recommendations. Predicted size of target protein is 48 kDa, detected band size is 55 kDa due to a His tag. 


    Binding activity of anti-nucleoprotein (N) antibodies in ELISA

    Wells were coated with Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) at 5 µg/ml and could bind anti- Nucleoprotein (N) antibodies with E50 of 43.50-118.4 ng/ml. 

  • Additional Information
  • Additional information (application): Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv were expressed in 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal. Therefore, anti-human IgG1 Fc, secondary antibody has to be used:
    AS10 791 | Anti-Human IgG Fc, HRP conjugated, goat antibodies
    AS10 797 | Anti-Human IgG Fc, HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum, goat antibodies
    AS10 787 | Anti-Human IgG Fc, ALP conjugated, goat antibodies
    AS10 793 | Anti-Human IgG Fc, ALP conjugated, min. cross-reactivity to bovine/mouse/rabbit serum, goat antibodies
  • Background
  • Background: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human) plays a fundamental role during virion assembly through packaging of the positive strand viral genome RNA into a helical ribonucleocapsid (RNP).

    Alternative names:
    NC, Protein N, Nucleocapsid protein
  • Protocols
  • Antibody protocols
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