Nucleoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV (human)
AS20 4387 | Clonality: Recombinant Monoclonal | Host: Mouse | Reactivity: Nucleaoprotein (N) of Novel Coronavirus SARS-CoV-2/ 2019-nCoV
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200 ng of His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein overexpressed in E. coli was separated on a 10 % SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5 % non-fat milk and following a series of washes with TBS-T and incubated with anti-SARS-CoV-2 nucleocapsid antibodies at a dilution of 1:1000 at RT for 2 h. Following a series of washes with TBS-T the membrane was incubated with a secondary antibody, goat anti-human IgG, Fc fragment at 1: 20 000 for 1h/RT. Following a standard series of washes with TBS-T, reaction was visualised with chemiluminescence following manufacture's recommendations. Predicted size of target protein is 48 kDa, detected band size is 55 kDa due to a His tag.
Wells were coated with Human Novel Coronavirus Nucleoprotein(N) (AS20 4388) at 5 µg/ml and could bind anti- Nucleoprotein (N) antibodies with E50 of 43.50-118.4 ng/ml.
AS10 791 | Anti-Human IgG Fc, HRP conjugated, goat antibodies
AS10 797 | Anti-Human IgG Fc, HRP conjugated, min. cross-reactivity bovine/mouse/rabbit serum, goat antibodies
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AS10 793 | Anti-Human IgG Fc, ALP conjugated, min. cross-reactivity to bovine/mouse/rabbit serum, goat antibodies
Alternative names: NC, Protein N, Nucleocapsid protein