Oxalate oxidase, Biotin conjugated
AS09 519 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Hordeum vulgare
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Product Information
Immunogen
native oxalate oxidase have been purified from barley seedlings
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG
Format
Lyophilized in phosphate buffer saline, labelled with biotin
Quantity
1 ml (10 mg/ml)
Reconstitution
For reconstitution please add 1 ml of sterile destilled water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 4000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW
21 kDa
Reactivity
Confirmed reactivity
Hordeum vulgare
Predicted reactivity
Triticum aestivum, Oryza sativa
Application examples
Additional information
Additional information
Biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidoBiotin.
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Background
Background
Oxalate oxidase (EC=1.2.3.4) belongs to the family of oxidoreductases, and acts on the aldehyde or oxo group of donor with oxygen as acceptor. Those reactions are specific forglyoxylate and dicarboxylate metabolism. Alternative names: oxygen oxidoreductase, aero-oxalo dehydrogenase, oxalic acid oxidase.
Product citations
Selected references
not available at the moment
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